IL-18 BPc Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-18 BPc Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑18 BPc
  Arg25-Ser192
  Accession # AAD17193
• Specificity: Detects mouse IL-18 BPc in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse IL-18 BPd is observed and less than 1% cross-reactivity with recombinant human IL-18 BPa is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il18r1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 BPc Antibody [Biotin]

• I;Il1;Il18r1;Il18ralpha;Il1rrp;Interleukin-18 receptor 1
• IL18 BPc
• IL-18 BPc

Background

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma , suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18 BPc Antibody (BAF129)(1)

Publication using BAF129

• Menachem, A;Alteber, Z;Cojocaru, G;Fridman Kfir, T;Blat, D;Leiderman, O;Galperin, M;Sever, L;Cohen, N;Cohen, K;Granit, RZ;Vols, S;Frenkel, M;Soffer, L;Meyer, K;Menachem, K;Galon Tilleman, H;Morein, D;Borukhov, I;Toporik, A;Perpinial Shahor, M;Tatirovsky, E;Mizrachi, A;Levy-Barda, A;Sadot, E;Strenov, Y;Eitan, R;Jakobson-Setton, A;Yanichkin, N;Ferre, P;Ophir, E; Unleashing Natural IL18 Activity Using an Anti-IL18BP Blocker Induces Potent Immune Stimulation and Antitumor Effects Cancer immunology research 2024-04-01 [PMID: 38592331] (ELISA Detection, Mouse)

Bioinformatics

• Gene Symbol: Il18r1
• Uniprot:
  • AAD17193()