IL-17RB Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-17RB Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB
  Arg18-Gly289
  Accession # NP_061195
• Specificity: Detects human IL‑17 RB in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse (rm) IL-17 RB is observed, 5% cross-reactivity with rmIL-17 R is observed, and less than 1% cross-reactivity with recombinant human IL-17 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17RB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RB Antibody [Biotin]

• CRL4IL-17 receptor homolog 1
• cytokine receptor CRL4
• Cytokine receptor-like 4
• Evi27
• EVI27IL-17B receptor
• IL-17 RB
• IL-17 receptor B
• IL-17 RH1
• IL17BRinterleukin 17B receptor
• IL-17ER
• IL17RB
• IL-17RB
• IL17Rh1
• IL-17Rh1
• IL17RH1MGC5245
• interleukin 17 receptor B
• interleukin 17 receptor homolog 1
• interleukin-17 receptor B
• Interleukin-17B receptor

Background

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Shi Y, et al. (2000) J. Biol. Chem. 275:19167.
3. Lee, J, et al. (2001) J. Biol. Chem. 276:1660.
4. Tian E, et al. (2000) Oncogene 19:2098.
5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
6. Hurst, S.D. et al. (2002) J. Immunol. 169:443.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RB Antibody (BAF1207)(1)

Publication using BAF1207

• Graeme Bredo, Jessica Storie, Nami Shrestha Palikhe, Courtney Davidson, Alexis Adams, Harissios Vliagoftis et al. Interleukin-25 initiates Th2 differentiation of human CD4(+) T cells and influences expression of its own receptor Immunity, Inflammation and Disease 2015-12-01 [PMID: 26734466]

Bioinformatics

• Gene Symbol: IL17RB
• Uniprot:
  • NP_061195()