Human IL-17RA/IL-17R ELISA Kit (Colorimetric) Summary
| Sensitivity |
10 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IL17RA |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
IL17RA (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL17RA. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent B (Item E): 5x concentrated buffer.
- Assay Diluent C (Item L): Diluent buffer.
- Detection Antibody IL-17R (Item F): Biotinylated anti-human IL-17R. Each vial isenough to assay the half microplate.
- HRP-Streptavidin Concentrate (Item G): 200x concentrated HRP-conjugatedstreptavidin.
- IL-17R Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-humanIL-17R.
- Standards Protein (Item C): Human IL-17R. 1 vial is enough to run each standard induplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB) inbuffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human IL-17RA/IL-17R ELISA Kit (Colorimetric)
Background
IL17RA is a gene that codes for a widely expressed protein that functions as a receptor for IL17A (a proinflammatory cytokine secreted by T-lymphocytes) with low affinity, which suggests that additional components are necessary to induce signaling and has a length of 866 amino acids and a weight of approximately 96 kDa. Current studies are being done on several diseases and disorders related to this gene including rheumatoid arthritis, cat eye syndrome, candidiasis, pituitary adenoma, bronchiolitis, cystic fibrosis, Sjogren's syndrome, keratitis, myocarditis, hepatitis B, liver disease, gingivitis, and osteoarthritis. IL17RA has also been shown to have interactions with IL17F, IL17RE, UBC, and TRAF3IP2 in pathways such as the IL-21 signaling, NF-kappaB signaling, and cytokine production pathways.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-17RA/IL-17R ELISA Kit (KA1734). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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