IL-17F Antibody (716728) [Unconjugated]

IL-17F was detected in immersion fixed Th17-differentiated rat splenocytes using Mouse Anti-Rat IL-17F Monoclonal Antibody (Catalog # MAB4437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Rt
• Applications: ICC/IF
• Clone: 716728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-17F Antibody (716728) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat IL-17F
  Ala27-Ala161
  Accession # NP_001015011
• Specificity: Detects rat IL-17F in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17F
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17F Antibody (716728) [Unconjugated]

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

Interleukin-17F (also ML-1) is a 19 kDa member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 2, 3, 4). Based on mouse, mature rat IL-17F is 133 amino acids (aa) in length (5, 6). Rat IL-17F is a presumably secreted, 38 kDa glycosylated disulfide-linked homodimer. It is also secreted as a 35 kDa disulfide-linked heterodimer with IL-17/17A (7, 8). The heterodimeric form represents about 30% of secreted IL-17F. Initially, IL-17F was also reported as IL-24. Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature rat IL-17F shares 59% and 90% aa sequence identity with mature human and mouse IL-17F, respectively; it also shares 55% aa identity with rat IL-17. Interspecies studies suggest rat IL-17F is produced by activated Th17-type CD4⁺ T cells, mast cells, basophils and monocytes (1, 3, 9), and is inducible through the interaction of TGF-beta , IL-6 and IL-23 (9, 10, 11). Targets for IL-17F include respiratory epithelium, fibroblasts, macrophages and endothelial cells which produce proinflammatory cytokines such as GM-CSF, IL-6, IFN-gamma , IP-10, MIP-1 alpha and MCP-1 (2, 6, 12). This activity is found for both homodimeric and heterodimeric forms of IL-17F (7).

1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
3. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:801.
4. Gaffen, S.H. et al. (2006) Vitam. Horm. 74:255.
5. Genbank Accession # : NP_001015011.
6. Oda, N. et al. (2005) Am. J. Respir. Cell Mol. Biol. 171:12.
7. Chang, S.H. and C. Gong (2007) Cell Res. 17:435.
8. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
9. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
10. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
11. Yang, X.O. et al. (2007) J. Biol. Chem. 282:9358.
12. Mcallister, F. et al. (2005) J. Immunol. 175:404.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL17F
• Uniprot:
  • NP_001015011()