IL-17F Antibody (716728) [Alexa Fluor® 488]

• Reactivity: Rt
• Applications: ICC/IF
• Clone: 716728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

IL-17F Antibody (716728) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant rat IL-17F
  Ala27-Ala161
  Accession # NP_001015011
• Specificity: Detects rat IL-17F in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17F Antibody (716728) [Alexa Fluor® 488]

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• interleukin 17F

Background

Interleukin-17F (also ML-1) is a 19 kDa member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 2, 3, 4). Based on mouse, mature rat IL-17F is 133 amino acids (aa) in length (5, 6). Rat IL-17F is a presumably secreted, 38 kDa glycosylated disulfide-linked homodimer. It is also secreted as a 35 kDa disulfide-linked heterodimer with IL-17/17A (7, 8). The heterodimeric form represents about 30% of secreted IL-17F. Initially, IL-17F was also reported as IL-24. Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature rat IL-17F shares 59% and 90% aa sequence identity with mature human and mouse IL-17F, respectively; it also shares 55% aa identity with rat IL-17. Interspecies studies suggest rat IL-17F is produced by activated Th17-type CD4⁺ T cells, mast cells, basophils and monocytes (1, 3, 9), and is inducible through the interaction of TGF-beta , IL-6 and IL-23 (9, 10, 11). Targets for IL-17F include respiratory epithelium, fibroblasts, macrophages and endothelial cells which produce proinflammatory cytokines such as GM-CSF, IL-6, IFN-gamma , IP-10, MIP-1 alpha and MCP-1 (2, 6, 12). This activity is found for both homodimeric and heterodimeric forms of IL-17F (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.