IL-17F Antibodies

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-17F was detected in immersion fixed mouse T helper 17 (Th17) cells stimulated with PMA and calcium ionomycin using Goat Anti-Mouse IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2057-IL) was serially diluted and captured by Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2057-IL) was serially diluted and captured by Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2057-IL) was serially diluted and captured by Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2057-IL) was serially diluted and captured by Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-17F was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human/Primate IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1335) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox"> VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_thumb" />

240-B), and 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), then stained with Rat Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB2057, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> 240-B), and 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), then stained with Rat Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB2057, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Mouse splenocytes were treated for 24 hr with 50 ng/mL PMA, 200 ng/mL Ca²⁺ionomycin, 10 ng/mL Recombinant Human TGF-beta (Catalog # 240-B), and 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), then stained with Rat Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB2057, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13351) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

M6000B). Under these conditions, IL-6 secretion elicited by IL-17F is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13352). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_lightbox"> M6000B). Under these conditions, IL-6 secretion elicited by IL-17F is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13352). The ND₅₀ is typically 0.1-0.6 µg/mL." alt="Recombinant Human IL-17F induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent response (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). Under these conditions, IL-6 secretion elicited by IL-17F is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13352). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_thumb" />

MAB13354) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB13354) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IL-17F protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13354) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111)." class="big_lightbox"> FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111)." alt="Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111)." class="big_thumb" />

DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IL-17F protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13354) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-17F was detected in immersion fixed Th17-differentiated rat splenocytes using Mouse Anti-Rat IL-17F Monoclonal Antibody (Catalog # MAB4437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />