IL-17F Antibody (4H1629)

Western Blot: IL-17F Antibody (4H1629) [NBP2-27327] - Analysis on full-length recombinant IL-17F proteins of (1) Human, (2) Mouse and (3) Rat using IL-17F antibody (clone 4H1629) at a concentration of 0.5 ug/ml. Note: This antibody did not detect the mouse or rat -IL-17F recombinant proteins.

Immunohistochemistry-Paraffin: IL-17F Antibody (4H1629) [NBP2-27327] - Detection IL-17F protein in a tissue section of human lymph node cancer using IL-17F antibody (clone 4H1629) at a concentration of 5 ug/ml.

Immunohistochemistry-Paraffin: IL-17F Antibody (4H1629) [NBP2-27327] - Analysis of IL-17F in formalin-fixed paraffin-embedded human colon tissue using an isotype control (top left) and IL-17F antibody (bottom left and right) at 5 ug/ml concentration.

Immunohistochemistry-Paraffin: IL-17F Antibody (4H1629) [NBP2-27327] - Detection -IL-17F protein in a tissue section of normal human stomach using IL-17F antibody (clone 4H1629) at a concentration of 5 ug/ml. Cytoplasmic expression was observed all over the tissue section and the staining was found to be more intense expecially in the chief cells of the glands.

Immunohistochemistry-Paraffin: IL-17F Antibody (4H1629) [NBP2-27327] - Detection IL17F protein in a tissue section of human stomach cancer using IL17F antibody (clone 4H1629) at a concentration of 5 ug/ml. Diffused cytoplasmic staining was observed in the tissue section and interestingly, the expression levels in cancerous tissue were found to be weaker than the parallel stained normal tissue section.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clone: 4H1629
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

IL-17F Antibody (4H1629) Summary

• Immunogen: Full-length recombinant MRkIPkVGHT FFQkPESCPP VPGGSMkLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGkEDISMN SVPIQQETLV VRRkHQGCSV SFQLEkVLVT VGCTCVTPVI HHVQ
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17F
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2ug/ml~
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml

Reactivity Notes

Note: The human IL17F protein shows 60% sequence similarity with Rat and 57% with Mouse, and in our testing this antibody did not react with Rat or Mouse recombinant IL17F proteins.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-17F Antibody (4H1629)

• Cytokine ML-1
• IL17F
• IL-17F
• IL-17Finterleukin-17F
• IL24
• IL-24
• interleukin 17F
• Interleukin-24
• ML-1
• ML1interleukin-24

Background

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-Beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterzed and highly expressed by activated effector memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL17F
• Entrez:
  • 112744(Human)
• Uniprot:
  • Q96PD4()