Human IL-17E/IL-25 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. IL25 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human IL25. |
| Standard Curve Range |
31.25 to 2000 pg/mL |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IL25 |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
Kit Components
|
Components
|
- 400x Streptavidin-HRP
- Adhesive Plate Sealers
- Assay Diluent
- Biotinylated Detection Antibody (Lyophilized)
- Microstrips Coated w/ Capture Antibody
- Protein Standard (Lyophilized)
- Ready-to-Use Substrate
- Stop Solution
- Wash Buffer (15x)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human IL-17E/IL-25 ELISA Kit (Colorimetric)
Background
Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for IL-17E/IL-25 ELISA Kit (KA2190)(1)
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Product General Protocols
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FAQs for IL-17E/IL-25 ELISA Kit (KA2190). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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