IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IL-17E/IL-25 Antibody (RM0263-5J46) [NBP2-11677] - Immunofluorescence for IL25-positive cells (red), actin (green) and nuclei (blue) in lung tissue from control mice. The control mice had no experimental manipulation performed. C57BL/6 male mice (Jackson Laboratories, Bar Harbor, Me), 8 to 12 weeks old and weighing 20 to 30 g, were used in this study. Samples were imaged on a FluoView 1000 confocal scanning microscope (Olympus). Imaging conditions were maintained at identical settings within each antibody-labeling experiment with original gating performed using the negative control (no primary antibody). Image courtesy of Center for Biologic Imaging, The University of Pittsburgh

Product Discontinued

IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free Summary

• Immunogen: Mouse recombinant IL-17E/IL-25
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: IL25
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:50-1:200
• Application Notes: Use in IHC-P reported in scientific literature (PMID:34571811). IF data from customer review.

Reactivity Notes

Use in Human reported in scientific literature (PMID:34571811). .

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for IL-17E/IL-25 Antibody (RM0263-5J46) - Azide and BSA Free

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17E/IL-25 Antibody (NBP2-11677)(1)

Publication using NBP2-11677

• Mehrara BJ, Park HJ, Kataru RP et al. Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema Biology 2021-09-18 [PMID: 34571811] (IHC-P, Human)

Review for IL-17E/IL-25 Antibody (NBP2-11677) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Bryan Tinsley IF Mouse 08/22/2014

Summary

• Application: Immunofluorescence
• Species: Mouse

Bioinformatics

• Gene Symbol: IL25
• Uniprot:
  • NP_542767()