IL-17D Antibodies

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin." alt="Mouse splenocytes were stained with Rat Anti-Mouse IL-17D Monoclonal Antibody (Catalog # MAB2274, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IL‑17D was detected in immersion fixed paraffin-embedded sections of human heart using 25 µg/mL Human IL‑17D Monoclonal Antibody (Catalog # MAB15041) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IL-17D was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human IL-17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1504) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB22741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB22741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Mouse IL‑17D protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (MAB22741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

2274-ML) was serially diluted and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB22741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2274-ML) was serially diluted and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB22741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Mouse IL‑17D protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB22741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

2274-ML) was serially diluted and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB22741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2274-ML) was serially diluted and captured by Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB22741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />