IL-17C Antibody (311523) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clone: 311523
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

IL-17C Antibody (311523) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑17C
  His15-Gln194
  Accession # NP_665833
• Specificity: Detects mouse IL-17C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17D, rmIL-17E, rmIL-17F, or recombinant human IL-17C is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17C Antibody (311523) [Alexa Fluor® 350]

• CX2
• Cytokine CX2
• IL17C
• IL-17C
• IL-17CMGC126884
• interleukin 17C
• interleukin-17C
• MGC138401

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1 ‑ 4). Mouse IL-17C cDNA encodes a 194 amino acid (aa) protein with a putative 14 aa signal peptide (5). Although there are no potential N-linked glycosylation sites, it is reportedly glycosylated (6). IL-17C shares from 15% ‑ 30% aa sequence identity with other IL-17 family members. Mouse and human IL-17C share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). IL-17C has been shown to stimulate the release of TNF-alpha and IL-1 beta from the monocytic cell line THP-1, a property it shares with IL-17B (6, 7). Human IL‑17C is active on mouse cells (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.