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VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox" target="_blank">
1234-IL) was serially diluted and captured by Mouse Anti-Human IL‑17C Monoclonal Antibody (Catalog # MAB1234) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑17C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1234) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
8358-MR) is immobilize at 0.06 µg/mL, Recombinant Human IL-17C (Catalog # 9640-IL) binds with an ED50 of 0.2-1.2 ng/mL." class="big_lightbox" target="_blank">
IL17C is encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. [provided by RefSeq]