IL-17/IL-17A Antibody (41802) [Unconjugated]

Western blot shows lysates of purified naïve human CD4+T cells orin vitrodifferentiated human Th17 CD4+ T cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-17 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB3171) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human IL-17 was immunoprecipitated from 100 µg of humanin vitrodifferentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171) or isotype control antibody (Catalog # MAB002) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Mouse Anti-Human IL‑17 Monoclonal Antibody (Catalog # MAB3171, dark orange filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, IHC, IP, CyTOF-ready, Flow
• Clone: 41802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-17/IL-17A Antibody (41802) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-17
  Ile20-Ala155
  Accession # Q16552
• Specificity: Detects human IL-17 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 0.5-25 ug/mL
  • Immunoprecipitation 3 ug/100 ug cell lysate
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (41802) [Unconjugated]

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17/IL-17A Antibody (MAB3171)(10)

Publications using MAB3171

• Lee, AR;Lee, SY;Choi, JW;Um, IG;Na, HS;Lee, JH;Cho, ML; Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model Experimental & molecular medicine 2023-08-01 [PMID: 37524866] (IHC, Confocal Imaging)
• Lee, AR;Lee, SY;Choi, JW;Um, IG;Na, HS;Lee, JH;Cho, ML; Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model Experimental & molecular medicine 2023-08-01 [PMID: 37524866] (IHC, Mouse)
• Seon-Yeong Lee, A Ram Lee, Jeong Won Choi, Chae Rim Lee, Keun-Hyung Cho, Jung Ho Lee et al. IL-17 Induces Autophagy Dysfunction to Promote Inflammatory Cell Death and Fibrosis in Keloid Fibroblasts via the STAT3 and HIF-1 alpha Dependent Signaling Pathways Frontiers in Immunology 6/10/2022 [PMID: 35757697]
• JW Bolt, AW van Kuijk, MBM Teunissen, D van der Co, S Aarrass, DM Gerlag, PP Tak, MG van de San, MC Lebre, LGM van Baarse Impact of Adalimumab Treatment on Interleukin-17 and Interleukin-17 Receptor Expression in Skin and Synovium of Psoriatic Arthritis Patients with Mild Psoriasis Biomedicines, 2022-01-29;10(2):. 2022-01-29 [PMID: 35203534] (IHC, Human)
• Rusan Ali Catar, Maria Bartosova, Edyta Kawka, Lei Chen, Iva Marinovic, Conghui Zhang et al. Angiogenic Role of Mesothelium-Derived Chemokine CXCL1 During Unfavorable Peritoneal Tissue Remodeling in Patients Receiving Peritoneal Dialysis as Renal Replacement Therapy Frontiers in Immunology 2/4/2022 [PMID: 35185912]
• MB Ismail, SO Åkefeldt, M Lourda, D Gavhed, R Gayet, M Aricò, JI Henter, C Delprat, H Valentin Comparison of three different ELISAs for the detection of recombinant, native and plasma IL-17A MethodsX, 2020-07-16;7(0):100997. 2020-07-16 [PMID: 32775222] (ELISA Capture, Human)
• Athanasios Arampatzioglou, Dimitrios Papazoglou, Theocharis Konstantinidis, Akrivi Chrysanthopoulou, Alexandros Mitsios, Iliana Angelidou et al. Clarithromycin Enhances the Antibacterial Activity and Wound Healing Capacity in Type 2 Diabetes Mellitus by Increasing LL-37 Load on Neutrophil Extracellular Traps Frontiers in Immunology 9/10/2018 [PMID: 30250474]
• Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129. 2012-07-11 [PMID: 22795370] (IHC, Human)
• Taiji Nakashima, Masatoshi Jinnin, Keitaro Yamane, Noritoshi Honda, Ikko Kajihara, Takamitsu Makino et al. Impaired IL-17 Signaling Pathway Contributes to the Increased Collagen Expression in Scleroderma Fibroblasts The Journal of Immunology 4/15/2012 [PMID: 22403442]
• Coury F, Annels N, Rivollier A, Olsson S, Santoro A, Speziani C, Azocar O, Flacher M, Djebali S, Tebib J, Brytting M, Egeler RM, Rabourdin-Combe C, Henter JI, Arico M, Delprat C Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion. Nat. Med., 2007-12-23;14(1):81-7. 2007-12-23 [PMID: 18157139] (IHC-P, Human)

FAQs for IL-17/IL-17A Antibody (MAB3171). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • Q16552()