IL-15R alpha Antibody [Unconjugated]

IL-15 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL‑15 Ra was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human IL‑15 R alpha Fc Chimera (Catalog #147‑IR) inhibits Recombinant Human IL‑15 (Catalog #247‑IL) induced proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑15 (2 ng/mL) activity elicited by Recombinant Human IL‑15 R alpha Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND50 is typically1‑4 µg/mL.

Measured levels of sIL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluids from RA and OA patients. Cytokine concentration of IL-15Ralpha (A), IL-15 (B), TNFalpha (C) and IL-6 (D) were measured in in duplicate SF samples from RA (n = 30) and OA (n = 30) by ELISA. Box plot represents media ± SD. Differences between two groups were performed by Mann–Whitney U test for nonparametric data. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25879761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for IL15Ra mRNA (ACD RNAScope Probe, catalog #532368; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human IL15Ra polyclonal antibody (R&D Systems catalog # AF247) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands.

• Reactivity: Hu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready, Dual ISH-IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-15R alpha Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human
  IL‑15 R alpha
  Ile31-Thr172
  Accession # EAW86418
• Specificity: Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human
  IL‑2 R gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL15RA
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-4 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-15R alpha Antibody [Unconjugated]

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.
IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of
IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha , IL-2 R beta , and IL-2 R gamma _c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15R alpha Antibody (AF247)(26)

Publications using AF247

• Kovalik, ME;Dacanay, MA;Crowley, SD;Hall, G; Swollen Feet: Considering the Paradoxical Roles of Interleukins in Nephrotic Syndrome Biomedicines 2024-03-26 [PMID: 38672094] (Immunohistochemistry, Human)
• Jaikumar Duraiswamy, Riccardo Turrini, Aspram Minasyan, David Barras, Isaac Crespo, Alizée J. Grimm et al. Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation Cancer Cell 2021-12-01 [PMID: 34739845]
• Catherine B. Xie, Bo Jiang, Lingfeng Qin, George Tellides, Nancy C. Kirkiles-Smith, Dan Jane-wit et al. Complement-activated interferon-gamma –primed human endothelium transpresents interleukin-15 to CD8+ T cells Journal of Clinical Investigation 2020-05-26 [PMID: 32191642]
• Agnès Quéméner, Sébastien Morisseau, Rui P. Sousa, Kilian Trillet, Mike Maillasson, Isabelle Leray et al. IL-15R alpha membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling Journal of Cell Science 2020-03-01 [PMID: 31653781]
• Qi‑Wen Man, Lin‑Zhou Zhang, Yi Zhao, Jin‑Yuan Liu, Yue‑Yu Zheng, Yi‑Fang Zhao et al. Lymphocyte‑derived microparticles stimulate osteoclastogenesis by inducing RANKL in fibroblasts of odontogenic keratocysts Oncology Reports 2018-09-18 [PMID: 30272301] (Neutralization, Human)
• Dionysios C. Watson, Defne Bayik, Avinash Srivatsan, Cristina Bergamaschi, Antonio Valentin, Gang Niu et al. Efficient production and enhanced tumor delivery of engineered extracellular vesicles Biomaterials 2016-10-01 [PMID: 27522254] (Western Blot, Human)
• M. Thaysen-Andersen, E. Chertova, C. Bergamaschi, E. S. X. Moh, O. Chertov, J. Roser et al. Recombinant human heterodimeric IL-15 complex displays extensive and reproducible N- and O-linked glycosylation Glycoconjugate Journal 2016-06-01 [PMID: 26563299]
• Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015-08-14;195(6):2818-28. 2015-08-14 [PMID: 26276870] (ICC, Neutralization, Human)
• Alicia Santos Savio, Ana Cecilia Machado Diaz, Araceli Chico Capote, Jamilet Miranda Navarro, Yunier Rodríguez Alvarez, Ricardo Bringas Pérez et al. Differential expression of pro-inflammatory cytokines IL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluid from Rheumatoid arthritis patients BMC Musculoskeletal Disorders 2015-12-01 [PMID: 25879761]
• Alexander Rölle, Julia Pollmann, Eva-Maria Ewen, Vu Thuy Khanh Le, Anne Halenius, Hartmut Hengel et al. IL-12–producing monocytes and HLA-E control HCMV-driven NKG2C+ NK cell expansion Journal of Clinical Investigation 2014-12-01 [PMID: 25384219]
• Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells. Gene Ther, 2014-10-02;22(1):76-86. 2014-10-02 [PMID: 25273353] (Western Blot, Human)
• Williams M, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman K, McAndrew R, Thomas A, Spence A, Gibson B, Graham G, Halsey C Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia. Blood, 2014-04-03;123(20):3116-27. 2014-04-03 [PMID: 24700781] (Neutralization, Human)
• Romero A, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, Caignard A, Messaoudene M, Auperin A, Vielh P, Dessen P, Porta C, Mateus C, Ayyoub M, Valmori D, Eggermont A, Robert C, Zitvogel L Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Ralpha and NKG2D triggering. Cancer Res, 2013-11-06;74(1):68-80. 2013-11-06 [PMID: 24197135] (IHC-P, Human)
• Muller J, Waldmann T, Kruhlak M, Dubois S Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells. J Biol Chem, 2012-10-16;287(48):40328-38. 2012-10-16 [PMID: 23074221] (ELISA Development, Human)
• Ana Cecilia Machado Diaz, Araceli Chico Capote, Celia Aurora Arrieta Aguero, Yunier Rodríguez Alvarez, Diana García del Barco Herrera, Miguel Estévez del Toro et al. Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid Arthritis Arthritis 2012-07-25 [PMID: 22888423]
• Jacques Banchereau, LuAnn Thompson-Snipes, Sandra Zurawski, Jean-Philippe Blanck, Yanying Cao, Sandra Clayton et al. The differential production of cytokines by human Langerhans cells and dermal CD14(+) DCs controls CTL priming Blood 2012-06-14 [PMID: 22535664]
• Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway. PLoS ONE, 2012-02-21;7(2):e31624. 2012-02-21 [PMID: 22363690] (Western Blot, Human)
• Jing Chen, Mike Petrus, Richard Bamford, Joanna H. Shih, John C. Morris, John E. Janik et al. Increased serum soluble IL-15R alpha levels in T-cell large granular lymphocyte leukemia Blood 2012-01-05 [PMID: 22049515]
• Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15. Blood, 2009-03-04;113(19):4541-7. 2009-03-04 [PMID: 19264676] (Flow Cytometry, Human)
• Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection. J. Immunol., 2009-02-01;182(3):1439-48. 2009-02-01 [PMID: 19155491] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops. J. Immunol., 2005-09-15;175(6):3656-62. 2005-09-15 [PMID: 16148110] (Neutralization, Human)
• Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A Renal cells express a functional interleukin-15 receptor. Nephrol. Dial. Transplant., 2005-01-25;20(3):516-23. 2005-01-25 [PMID: 15671076] (Western Blot, Human, Mouse)
• Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs. Proc. Natl. Acad. Sci. U.S.A., 2004-11-09;101(47):16606-11. 2004-11-09 [PMID: 15536127] (Flow Cytometry, Human)
• Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling. Leuk. Res., 2004-10-01;28(10):1023-31. 2004-10-01 [PMID: 15289013] (Neutralization, Human)
• Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. J. Immunol., 2004-07-15;173(2):1463-76. 2004-07-15 [PMID: 15240743] (Neutralization, Human)
• Hessian PA, Highton J, Kean A, Sun CK, Chin M Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Arthritis Rheum., 2003-02-01;48(2):334-8. 2003-02-01 [PMID: 12571841] (IHC, Human)

Bioinformatics

• Gene Symbol: IL15RA
• Uniprot:
  • EAW86418()