IL-15R alpha Antibody (151307) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 151307
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-15R alpha Antibody (151307) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑15 R alpha
  Ile31-Thr172
  Accession # EAW86418
• Specificity: Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑15 R alpha , recombinant human (rh) IL-2 R alpha , rhIL-2 R beta , or rhIL-2 R gamma is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL15RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-15R alpha Antibody (151307) [Unconjugated]

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.
IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of
IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha , IL-2 R beta , and IL-2 R gamma _c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15R alpha Antibody (MAB147)(5)

Publications using MAB147

• R Ciccociopp, A Gallia, MA Avanzini, E Betti, C Picone, A Vanoli, C Paganini, F Biagi, R Maccario, GR Corazza A Refractory Celiac Patient Successfully Treated With Mesenchymal Stem Cell Infusions Mayo Clin Proc, 2016-04-14;0(0):. 2016-04-14 [PMID: 27087453] (Western Blot, Human)
• Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A IL15RA drives antagonistic mechanisms of cancer development and immune control in lymphocyte-enriched triple-negative breast cancers. Cancer Res, 2014-06-30;74(17):4908-21. 2014-06-30 [PMID: 24980552] (IHC-P, Human)
• Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. PLoS ONE, 2012-07-09;7(7):e40620. 2012-07-09 [PMID: 22792388] (Flow Cytometry, Human)
• Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15. Blood, 2009-03-04;113(19):4541-7. 2009-03-04 [PMID: 19264676] (Flow Cytometry, Western Blot, Human)
• Elliott SL, Suhrbier A, Miles JJ Phase I trial of a CD8+ T-cell peptide epitope-based vaccine for infectious mononucleosis. J. Virol., 2007-11-21;82(3):1448-57. 2007-11-21 [PMID: 18032491] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL15RA
• Uniprot:
  • EAW86418()