Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

ELISA: IL-15 ELISA Kit [NBP1-92667]

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA), buffered solution
• Standard Curve Range: 6.3 - 400 pg/mL
• Sensitivity: 2.0 pg/mL

Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric) Summary

• Description: The mouse IL-15/IL-15R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-15/IL-15R.
• Specificity: Recombinant mouse IL-15 has a cross reactivity of 2% in this assay when spiked in excess (100 ng/ml). The specificity of this ELISA has been confirmed with bone-marrow derived dendritic cells from IL-15 knock-out mice; Tissue culture supernatant from these cells following lipopolysaccharide (LPS) stimulation was negative for the presence of IL-15/IL-15R complex while wild type animals reveal more than 40 pg/ml.
• Standard Curve Range: 6.3 - 400 pg/mL
• Sensitivity: 2.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.3%
• Intra-Assay: CV% < 5.3%
• Spike Recovery: Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL15

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

• IL-15/IL-15 R alpha Complex
• IL-15/IL-15R alpha Complex

Background

IL-15 is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15Ralpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans- presented to cells expressing the beta-gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10- 100-fold increase in agonistic activity over IL-15 alone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-15/IL-15R alpha Complex ELISA Kit (NBP1-92667)(2)

Publications using NBP1-92667

• Stroganov S Hyaluronan-NK cell Interaction Controls the Primary Vascular Barrier during Early Pregnancy bioRxiv 2020-01-01 (ELISA, Mouse)
• Hadas R, Gershon E, Cohen A et al. Hyaluronan control of the primary vascular barrier during early mouse pregnancy is mediated by uterine NK cells JCI Insight 2020-11-19 [PMID: 33208556] (ELISA)

FAQs for IL-15/IL-15R alpha Complex ELISA Kit (NBP1-92667). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL15