Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

ELISA: IL-15 ELISA Kit [NBP1-92667]

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA), buffered solution
• Standard Curve Range: 6.3 - 400 pg/mL
• Sensitivity: 2.0 pg/mL

Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric) Summary

• Description: The mouse IL-15/IL-15R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-15/IL-15R.
• Specificity: Recombinant mouse IL-15 has a cross reactivity of 2% in this assay when spiked in excess (100 ng/ml). The specificity of this ELISA has been confirmed with bone-marrow derived dendritic cells from IL-15 knock-out mice; Tissue culture supernatant from these cells following lipopolysaccharide (LPS) stimulation was negative for the presence of IL-15/IL-15R complex while wild type animals reveal more than 40 pg/ml.
• Standard Curve Range: 6.3 - 400 pg/mL
• Sensitivity: 2.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.3%
• Intra-Assay: CV% < 5.3%
• Spike Recovery: Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL15

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

• IL-15/IL-15 R alpha Complex
• IL-15/IL-15R alpha Complex

Background

IL-15 is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15Ralpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans- presented to cells expressing the beta-gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10- 100-fold increase in agonistic activity over IL-15 alone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-15/IL-15R alpha Complex ELISA Kit (NBP1-92667)(2)

Publications using NBP1-92667

• Stroganov S Hyaluronan-NK cell Interaction Controls the Primary Vascular Barrier during Early Pregnancy bioRxiv 2020-01-01 (ELISA, Mouse)
• Hadas R, Gershon E, Cohen A et al. Hyaluronan control of the primary vascular barrier during early mouse pregnancy is mediated by uterine NK cells JCI Insight 2020-11-19 [PMID: 33208556] (ELISA)

FAQs for IL-15/IL-15R alpha Complex ELISA Kit (NBP1-92667). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL15