Home » IL-12 p70/IL-12A » IL12A Research Products » IL-12 p70/IL-12A Antibody

IL-12 p70/IL-12A Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: IL-12 p70/IL-12A Antibody [NBP2-24627] - Analysis of IL-12A in recombinant IL-35 using IL-12A antibody at 4 ug/ml.
Western Blot: IL-12 p70/IL-12A Antibody [NBP2-24627] - Analysis of IL-12A in recombinant IL-35 using IL-12A antibody at 4 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Ca, Mk, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

100% Guarantee on every product

IL-12 p70/IL-12A Antibody Summary

Immunogen
A portion of amino acids 200-250 of IL-12A was used as the immunogen for this antibody.
Predicted Species
Mouse (95%), Primate (100%), Canine (95%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL12A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 3-5 ug/ml

Reactivity Notes

Predicted to react with goat, pig.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for IL-12 p70/IL-12A Antibody

  • CLMF p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL12 p70
  • IL-12 p70
  • IL-12 subunit p35
  • IL-12, subunit p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cdk5 Antibody (4E4)
NBP2-37602
Western Blot: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
Immunocytochemistry/Immunofluorescence: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of GC7901 cells using CDK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of cerebellum tissues (left) and brain tissues (right) using CDK5 mouse mAb with DAB staining.
Species: Hu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
ERK2 Antibody
(SZ25-01)
NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
p63/TP73L Antibody
[Unconjugated]
AF1916
p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Reviews)
Species: Hu
Applications: WB, IHC, ICC
IL-9
409-ML/CF
IL-9
(6 Publications)
Species: Mu
GEF-H1 Antibody
NBP2-21577
Western Blot: GEF-H1 Antibody [NBP2-21577] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody [NBP2-21577] - HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: ARHGEF2 protein stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Frozen: GEF-H1 Antibody [NBP2-21577] - Frozen-sectioned mouse mouse cerebellum. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
EBNA1BP2 Antibody
NBP1-84022
Western Blot: EBNA1BP2 Antibody [NBP1-84022] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: EBNA1BP2 Antibody [NBP1-84022] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EBNA1BP2 Antibody [NBP1-84022] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
LANCL1 Antibody
NBP1-81796
Western Blot: LANCL1 Antibody [NBP1-81796] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: LANCL1 Antibody [NBP1-81796] - Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
Immunohistochemistry-Paraffin: LANCL1 Antibody [NBP1-81796] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
p40 Antibody
NBP1-32956
Western Blot: p40 (p63 delta) Antibody [NBP1-32956] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: p40 (p63 delta) Antibody [NBP1-32956] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: p40 (p63 delta) Antibody [NBP1-32956] - A549 xenograft, using p40 antibody at 1:100 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
RPSA Antibody
(JF0955)
NBP2-67117
Western Blot: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: A431
Immunocytochemistry/Immunofluorescence: RPSA Antibody (JF0955) [NBP2-67117] - Staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
PSMD7 Antibody
NBP2-38780
Western Blot: PSMD7 Antibody [NBP2-38780] - Analysis using Anti-PSMD7 antibody NBP2-38780 (A) shows similar pattern to independent antibody NBP2-38612 (B).
Immunocytochemistry/Immunofluorescence: PSMD7 Antibody [NBP2-38780] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PSMD7 Antibody [NBP2-38780] - Staining of human kidney.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
CDK5 Activator 1
Antibody
AF7250
CDK5 Activator 1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human CDK5 Activator 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7250) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent‑Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei in neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: IHC
IL-23A/IL-23 P19
[Unconjugated]
1887-ML
IL-23A/IL-23 P19 [Unconjugated]
(4 Reviews)
(138 Publications)
Species: Mu
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(13 Reviews)
(316 Publications)
Species: Hu
TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(24 Reviews)
(622 Publications)
Species: Hu
TPPP/p25 Antibody
NBP1-49833
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
Lipocalin-2/NGAL
Antibody [Unconjugate ...
AF1857
Lipocalin‑2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected forLipocalin‑2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-MouseLipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(4 Reviews)
(35 Publications)
Species: Mu
Applications: WB, Simple Western, IHC, IP
TMED9 Antibody
NBP1-31650
Western Blot: TMED9 Antibody [NBP1-31650] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - Sample: Paraffin-embedded mouse duodenum. TMED9 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
p19ARF/CDKN2A
Antibody
NB200-106
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of FFPE mouse spleen using p16INK4a/CDKN2A antibody at 10 ug/mL on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
(1 Review)
(42 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Caspase-3 Antibody
(31A1067) - (Pro and ...
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
(1 Review)
(140 Publications)
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
IL-12 p70/IL-12A
Antibody
NBP2-24627
IL-12 p70/IL-12A Antibody
Species: Hu, Mu, Ca, Mk, Pm
Applications: WB
Supplier Logo

Publications for IL-12 p70/IL-12A Antibody (NBP2-24627) (0)

There are no publications for IL-12 p70/IL-12A Antibody (NBP2-24627).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-12 p70/IL-12A Antibody (NBP2-24627) (0)

There are no reviews for IL-12 p70/IL-12A Antibody (NBP2-24627). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-12 p70/IL-12A Antibody (NBP2-24627). (Showing 1 - 1 of 1 FAQ).

  1. May I request a copy of the WB protocol that was used to generate the WB image presented on the data sheet for NBP2-24627 (IL12A Antibody)?
    • This image was obtained using our standard Western blot protocol with the antibody used at a concentration of 4ug/mL.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional IL-12 p70/IL-12A Products

Bioinformatics Tool for IL-12 p70/IL-12A Antibody (NBP2-24627)

Discover related pathways, diseases and genes to IL-12 p70/IL-12A Antibody (NBP2-24627). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-12 p70/IL-12A Antibody (NBP2-24627)

Discover more about diseases related to IL-12 p70/IL-12A Antibody (NBP2-24627).
 

Pathways for IL-12 p70/IL-12A Antibody (NBP2-24627)

View related products by pathway.

PTMs for IL-12 p70/IL-12A Antibody (NBP2-24627)

Learn more about PTMs related to IL-12 p70/IL-12A Antibody (NBP2-24627).
 

Research Areas for IL-12 p70/IL-12A Antibody (NBP2-24627)

Find related products by research area.

Blogs on IL-12 p70/IL-12A

There are no specific blogs for IL-12 p70/IL-12A, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-12 p70/IL-12A Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol IL12A
Novus 100% Guarantee