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VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219). The ND50 is typically 0.3-0.9 µg/mL." class="big_lightbox" target="_blank">
Interleukin-12, or IL-12, also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine that affects both natural killer (NK) cells and T lymphocytes. IL-12 is a heterodimer of subunit IL-12A p35 and subunit IL-12B p40 that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. It is involved in mediating many aspects of antiviral immunity. In addition to stimulating T helper cell development, IL-12 is a potent inducer of interferon-gamma (IFN-gamma) production by T cells and NK cells. Increased IL-12 production is a consequence of EBV, CMV, LCMV or HSV infection. While it is unclear what separate effects can be attributed to each IL-12 subunit, evidence suggests IL-12A p35 is the biologically active component responsible for signal transduction via the IL-12 receptor.
![Immunohistochemistry IL-12 p70/IL-12A Antibody (24910) [Unconjugated]](http://images.novusbio.com/images/antibody/IL12_p70_MAB219_Immunohistochemistry_23318.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize/elisa/IL-12_M1270_ELISA_225.jpg)
![N/A IL-12 p70/IL-12A [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL12_p70_DY419_ELISA_2016.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL12_p70_D1200_ELISA_531.jpg)
![Neutralization IL-12 p70/IL-12A Antibody (24910) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-12_p70_MAB219_Block_Neutralize_2099.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize/elisa/m1270_mouse-il-12-p70-quantikine-elisa-kit-2-plate-1392021111415.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL12_p70_D1200_ELISA_532.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize/elisa/IL-12_D1200_ELISA_11.jpg)
