Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

ELISA: Human IL12B ELISA Kit (Colorimetric) [NBP1-82421] - Representative standard curve for human IL12B ELISA

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution
• Standard Curve Range: 31.3 - 2000 pg/mL
• Sensitivity: < 10 pg/mL

Human IL-12/IL-23 p40 ELISA Kit (Colorimetric) Summary

• Specificity: The assay detects both natural and recombinant human IL12B. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations. There was no crossreactivity observed with IL-1 beta, IL- 2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN gamma, TNF alpha and TNF beta. BMS2013 also detects IL/23and IL12p70.
• Standard Curve Range: 31.3 - 2000 pg/mL
• Sensitivity: < 10 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.4%
• Intra-Assay: CV% < 4.7%
• Spike Recovery: The recovery ranged from 65% to 75% with an overall mean recovery of 71%.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL12B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. P35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL12B