IL-12 Antibody [Unconjugated]

Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND50 is typically 0.01-0.06 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-12 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
  Mouse IL-12 heterodimer: Mouse IL-12 p40 (Met23-Ser335) Accession # P43432, Mouse IL-12 p35 (Arg23-Ala215) Accession # NP_032377
• Specificity: Detects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant human IL-12.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL12A
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.01-0.06 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 Antibody [Unconjugated]

• CLMF p35
• CLMF1
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL-12 subunit p35
• IL12
• IL-12
• IL-12, subunit p35
• IL12A
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159‑A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 Antibody (AF-419-NA)(17)

Publications using AF-419-NA

• Xiong, X;Huang, W;Yang, X;Wang, X;Wu, B;Li, D; Calycosin suppresses the activating effect of granulocyte-macrophage-colony-stimulating factor-producing T helper cells on macrophages in experimental atherosclerosis Frontiers in pharmacology 2025-07-10 [PMID: 40709089] (Neutralization, Mouse)
• Zhong, X;Wu, H;Zhang, W;Shi, Y;Gwack, Y;Xue, HH;Sun, Z; Distinct ROR?t-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infection Cell reports 2024-11-05 [PMID: 39504243] (Neutralization, Mouse)
• Blanco, T;Nakagawa, H;Musayeva, A;Krauthammer, M;Singh, RB;Narimatsu, A;Ge, H;Shoushtari, SI;Dana, R; Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation JCI insight 2024-09-05 [PMID: 39235864] (Bioassay, Mouse)
• Demo Yemane Tesfaye, Sonja Bobic, Anna Lysén, Peter Csaba Huszthy, Arnar Gudjonsson, Ranveig Braathen et al. Targeting Xcr1 on Dendritic Cells Rapidly Induce Th1-Associated Immune Responses That Contribute to Protection Against Influenza Infection Frontiers in Immunology 2/28/2022 [PMID: 35296089]
• Lijuan Sun, Tim Kees, Ana Santos Almeida, Bodu Liu, Xue-Yan He, David Ng et al. Activating a collaborative innate-adaptive immune response to control metastasis Cancer Cell 10/1/2021 [PMID: 34478639]
• Kun Gao, Jingmiao Jin, Chenyang Huang, Jianhang Li, Haihua Luo, Lei Li et al. Exosomes Derived From Septic Mouse Serum Modulate Immune Responses via Exosome-Associated Cytokines Frontiers in Immunology 2019-07-12 [PMID: 31354717] (Neutralization, Mouse)
• Kun Gao, Jingmiao Jin, Chenyang Huang, Jianhang Li, Haihua Luo, Lei Li et al. Exosomes Derived From Septic Mouse Serum Modulate Immune Responses via Exosome-Associated Cytokines Frontiers in Immunology 7/12/2019 [PMID: 31354717]
• Christopher D. Zahm, Viswa T. Colluru, Sean J. McIlwain, Irene M. Ong, Douglas G. McNeel TLR Stimulation during T-cell Activation Lowers PD-1 Expression on CD8+ T Cells Cancer Immunology Research 11/1/2018 [PMID: 30201735]
• Y Manome, D Suzuki, A Mochizuki, E Saito, K Sasa, K Yoshimura, T Inoue, M Takami, K Inagaki, T Funatsu, R Kamijo The inhibition of malignant melanoma cell invasion of bone by the TLR7 agonist R848 is dependent upon pro-inflammatory cytokines produced by bone marrow macrophages Oncotarget, 2018-07-06;9(52):29934-29943. 7/6/2018 [PMID: 30042824]
• W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang Crucial role of OX40/OX40L signaling in a murine model of asthma Mol Med Rep, 2018-01-18;0(0):. 2018-01-18 [PMID: 29344664] (Functional Assay, Mouse)
• Lorenzo C, Perez-Chacon G, Garaulet G, Mallorquin Z, Zapata J, Rodriguez A Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines. Cancer Gene Ther, 2015-10-09;22(11):542-51. 2015-10-09 [PMID: 26450626] (Western Blot, Mouse)
• Rashmi Jalah, Margherita Rosati, Brunda Ganneru, Guy R. Pilkington, Antonio Valentin, Viraj Kulkarni et al. The p40 subunit of interleukin (IL)-12 promotes stabilization and export of the p35 subunit: implications for improved IL-12 cytokine production Journal of Biological Chemistry 3/1/2013 [PMID: 23297419]
• Chinnery , Fay, King , Catherin, Elliott , Tim, Bateman , Andrew R, James , Edward Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk. Oncoimmunology, 2012-09-01;1(6):874-883. 2012-09-01 [PMID: 23162755] (Neutralization, Mouse)
• Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss. J. Immunol., 2010-12-13;186(2):869-77. 2010-12-13 [PMID: 21149611] (Neutralization, Mouse)
• Liu J, Dolan MC, Reidy M, Cramer CL Expression of bioactive single-chain murine IL-12 in transgenic plants. J. Interferon Cytokine Res., 2008-06-01;28(0):381-92. 2008-06-01 [PMID: 18593333] (Western Blot, Mouse)
• Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells. Immunol. Lett., 2007-12-17;116(1):86-94. 2007-12-17 [PMID: 18166232] (Neutralization, Mouse)
• Hajishengallis G, Shakhatreh MA, Wang M, Liang S Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo. J. Immunol., 2007-08-15;179(4):2359-67. 2007-08-15 [PMID: 17675497] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL12A