IL-1 RII Antibody

Images

 
Western Blot: IL-1 RII Antibody [NBP1-32681] - Sample (30 ug of whole cell lysate)A: 293T 10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: IL-1 RII Antibody [NBP1-32681] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Paraffin-embedded human colon cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500.
Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Paraffin-embedded human lung cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IL-1 RII Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IL1 Receptor 2. The exact sequence is proprietary.
Localization
Membrane, Single-pass type I membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1R2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-32681 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-1 RII Antibody

  • beta
  • CD121b antigen
  • CD121b
  • IL-1 R beta
  • IL-1 RII
  • IL1R2
  • IL1RBCD121 antigen-like family member B
  • IL-1R-beta
  • IL1RII
  • IL-1RII
  • IL-1RT2
  • IL-1RT-2
  • interleukin 1 receptor, type II
  • Interleukin-1 receptor beta
  • MGC47725

Background

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 RII Antibody (NBP1-32681)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Human
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL1R2
Entrez