IL-1 RII Antibody - BSA Free

Western Blot: IL-1 RII Antibody [NBP1-32681] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: IL-1 RII Antibody [NBP1-32681] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.

Immunohistochemistry: IL-1 RII Antibody [NBP1-32681] - Representative photomicrographs of IL-1beta receptor immunofluorescence staining of the cingulum (Bregma - 1.0) on P4. Image collected and cropped by CiteAb from the following publication (//bmcneurosci.biomedcentral.com/articles/10.1186/s12868-019-0529-1), licensed under a CC-BY license.

Chromatin Immunoprecipitation: IL-1 RII Antibody [NBP1-32681] - The LPS-induced changes in the levels of inflammatory cytokines in circulation and the brain tissue of rats on P4 (IL-1beta ). ** and *represent significant differences between groups (P < 0.01 and P < 0.05, respectively). ANOVA with a Bonferroni test was conducted. There were six rats per group Image collected and cropped by CiteAb from the following publication (//bmcneurosci.biomedcentral.com/articles/10.1186/s12868-019-0529-1), licensed under a CC-BY license.

Western Blot: IL-1 RII Antibody [NBP1-32681] - Sample (30 ug of whole cell lysate)A: 293T 10% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Human lung cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Human colon cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IL-1 RII Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IL-1 RII. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Predicted Species: Rhesus Macaque (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL1R2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for IL-1 RII Antibody - BSA Free

• beta
• CD121b antigen
• CD121b
• IL-1 R beta
• IL-1 RII
• IL1R2
• IL1RBCD121 antigen-like family member B
• IL-1R-beta
• IL1RII
• IL-1RII
• IL-1RT2
• IL-1RT-2
• interleukin 1 receptor, type II
• Interleukin-1 receptor beta
• MGC47725

Background

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IL-1 RII Antibody (NBP1-32681)(2)

Publications using NBP1-32681

• Shin SH, Kim EK, Lee KY, Kim HS TNF-alpha antagonist attenuates systemic lipopolysaccharide-induced brain white matter injury in neonatal rats BMC Neurosci 2019-08-30 [PMID: 31470812] (ICC/IF, Rat)
• Asaoka T, IslER, Tryphonopoulos P, Selvaggi G, Moon J, Tekin A, Amador A, Levi DM, Garcia J, Smith L, Nishida S, Weppler D, Tzakis AG, Ruiz P. Characteristic immune, apoptosis and inflammatory gene profiles associated with intestinal acute cellular rejection in formalin-fixed paraffin-embedded mucosal biopsies. Transpl Int;24(7):697-707. 2011-07-01 [PMID: 21557779] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: IL1R2