IL-1 RAcP/IL-1 R3 Antibody (2A3)

Immunoprecipitation: IL-1 RAcP/IL-1 R3 Antibody (2A3) [H00003556-M06] - Analysis of IL1RAP transfected lysate using anti-IL1RAP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1RAP rabbit polyclonal antibody.

ELISA: IL-1 RAcP/IL-1 R3 Antibody (2A3) [H00003556-M06] - Detection limit for recombinant GST tagged IL1RAP is 0.3 ng/ml as a capture antibody.

Product Discontinued

IL-1 RAcP/IL-1 R3 Antibody (2A3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IL1RAP (AAH53621.1, 1 a.a. ~ 356 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLLWCVVSLYFYGILQSDASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTPTVKVVGSPKNAVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQKGNRCGQ
• Specificity: IL1RAP - interleukin 1 receptor accessory protein (2A3)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1RAP
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IL-1 RAcP/IL-1 R3 Antibody (2A3)

• C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
• FLJ37788
• IL-1 R3
• IL-1 RAcP
• IL-1 receptor accessory protein
• IL-1R3
• IL-1R-3
• IL1RAcP
• IL-1RAcP
• IL1RAP
• interleukin 1 receptor accessory protein
• interleukin-1 receptor accessory protein beta
• interleukin-1 receptor accessory protein

Background

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes an interleukin 1 receptor accessory protein. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RAcP/IL-1 R3 Antibody (H00003556-M06)(1)

Publication using H00003556-M06

• Mitchell K, Barreyro L, Todorova TI et al. IL1RAP potentiates multiple oncogenic signaling pathways in AML. J Exp Med 2018-05-17 [PMID: 29773641]

Bioinformatics

• Gene Symbol: IL1RAP
• Entrez:
  • 3556(Human)
• Uniprot:
  • AAH53621(Human)