IL-1 beta/IL-1F2 Antibody (8516) [Unconjugated]

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant HumanIL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201). The ND50 is typically 0.001-0.003 µg/mL.

IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, B/N, CyTOF-ready, Flow, ICC/IF
• Clone: 8516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody (8516) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-1 beta /IL-1F2
  aa 117-269
  Accession # P01584
• Specificity: Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.
• Details of Functionality: ActivityAssaywCitation not found None None None None
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.001-0.003 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (8516) [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (MAB201)(26)

Publications using MAB201

• R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019;10(1):5016. 2019 [PMID: 31676788] (Neutralization, Human)
• K Vogel, M Pierau, A Arra, K Lampe, D Schlueter, C Arens, MC Brunner-We Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus Sci Rep, 2018;8(1):16904. 2018 [PMID: 30442915] (Neutralization, Human)
• DM Clancy, GP Sullivan, HBT Moran, CM Henry, EP Reeves, NG McElvaney, EC Lavelle, SJ Martin Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing Cell Rep, 2018;22(11):2937-2950. 2018 [PMID: 29539422] (Western Blot, Human)
• SEJ Chambers, CL O'Neill, J Guduric-Fu, KJ McLoughlin, A Liew, AM Egan, T O'Brien, AW Stitt, RJ Medina The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1? Stem Cells, 2018;36(6):834-843. 2018 [PMID: 29484768] (Western Blot, Human)
• Y Uchino, AM Woodward, J Mauris, K Peterson, P Verma, UJ Nilsson, J Rajaiya, P Argüeso Galectin-3 is an amplifier of the interleukin-1?-mediated inflammatory response in corneal keratinocytes Immunology, 2018;0(0):. 2018 [PMID: 29359328] (Western Blot, Human)
• F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression Cancer Res., 2017;0(0):. 2017 [PMID: 28754674] (Neutralization, Human)
• N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific Front Immunol, 2017;8(0):379. 2017 [PMID: 28421077] (Western Blot, Mouse)
• E Naschberge, W Gei beta dörfer, C Bogdan, P Tripal, E Kremmer, M Stürzl, N Britzen-La Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28272793] (Western Blot, Human)
• M Garstkiewi, GE Strittmatt, S Grossi, J Sand, G Fenini, S Werner, LE French, HD Beer Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28247911]
• Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability Sci Rep, 2016;6(0):39035. 2016 [PMID: 27976724] (Western Blot, Human)
• R A Steinman Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals Breast Cancer Res. Treat., 2016;0(0):. 2016 [PMID: 27853906] (ICC, Human)
• NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis Arch Med Sci, 2016;12(5):950-958. 2016 [PMID: 27695484] (Western Blot, Human)
• Wan-Chien Cheng Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Eur J Immunol, 2016;0(0):. 2016 [PMID: 27334899] (Neutralization, Human)
• Oliver R, Lovric V, Yu Y, Christou C, Aiken S, Cooper J, Walsh W Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue. PLoS ONE, 2015;10(8):e0136514. 2015 [PMID: 26305692] (IHC-P, Mouse)
• Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium. J Biol Chem, 2015;290(40):24067-78. 2015 [PMID: 26269588] (Neutralization, Human)
• Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh V, Chandran B Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-beta Responses. PLoS Pathog, 2015;11(7):e1005019. 2015 [PMID: 26134128] (Western Blot, Human)
• Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012;10(9):e1001395. 2012 [PMID: 23055831] (Neutralization, Human)
• Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012;7(7):e39871. 2012 [PMID: 22802946] (Neutralization, Human)
• Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Neutralization, Human)
• Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Eur. J. Immunol., 2009;39(5):1221-30. 2009 [PMID: 19350551] (Neutralization, Human)
• Li J, Yin HL, Yuan J Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity. J. Cell Biol., 2008;181(2):321-33. 2008 [PMID: 18411310] (Western Blot, Human)
• Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol., 2007;8(9):942-9. 2007 [PMID: 17676045] (Neutralization, Human)
• van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007;52(10):2775-83. 2007 [PMID: 17394066] (Neutralization, Human)
• Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway. Am. J. Respir. Cell Mol. Biol., 2006;35(4):407-14. 2006 [PMID: 16690986] (Neutralization, Human)
• Banks WA, Farr SA, La Scola ME Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum. J. Pharmacol. Exp. Ther., 2001;299(2):536-41. 2001 [PMID: 11602664] (Neutralization, Human)
• Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia. J. Neurochem., 2000;74(3):1017-25. 2000 [PMID: 10693932] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P01584()