IL-1 beta/IL-1F2 Antibody (8516) [Phycoerythrin]

Human peripheral blood monocytes stimulated with LPS were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human IL-1 beta /IL-1F2 PE-conjugated Monoclonal Antibody (Catalog # IC201P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 8516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-1 beta/IL-1F2 Antibody (8516) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human IL-1 beta /IL-1F2
  aa 117-269
  Accession # P01584
• Specificity: Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and recombinant porcine IL‑1 beta and no cross-reactivity with recombinant rat (rr) IL‑1 beta , rmIL‑1 alpha , recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (8516) [Phycoerythrin]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (IC201P)(6)

Publications using IC201P

• Zhu, A;Chen, Z;Yan, Q;Jiang, M;Liu, X;Li, Z;Li, N;Tang, C;Jian, W;He, J;Chen, L;Cheng, J;Chen, C;Tang, T;Xu, Z;Hu, Q;Li, F;Wang, Y;Sun, J;Zhuang, Z;Wen, L;Zhuo, J;Liu, D;Zhang, Y;Huang, X;Li, S;Zeng, Q;Chen, F;Zhou, L;Liu, D;Zhong, C;Chen, Y;Li, S;Liang, K;Zhong, N;Zhang, X;Chen, J;Chen, X;Xu, Y;Zhong, N;Zhao, J;Zhao, J; Robust mucosal SARS-CoV-2-specific T cells effectively combat COVID-19 and establish polyfunctional resident memory in patient lungs Nature immunology 2025-01-28 [PMID: 39875584] (Flow Cytometry, Human)
• Li T, Yang Y, Li Y et al. Platelets mediate inflammatory monocyte activation by SARS-CoV-2 Spike protein The Journal of clinical investigation 12/29/2021 [PMID: 34964720]
• Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli. J. Immunol., 2011-05-04;186(12):6701-9. 2011-05-04 [PMID: 21543643] (Flow Cytometry, Human)
• Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients. Cancer Res., 2007-10-15;67(20):9630-6. 2007-10-15 [PMID: 17942891] (Flow Cytometry, Human)
• Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). J. Invest. Dermatol., 2002-10-01;119(4):842-9. 2002-10-01 [PMID: 12406329] (Flow Cytometry, Human)
• Wang L, Das H, Kamath A, Li L, Bukowski JF Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production. J. Immunol., 2002-05-15;168(10):4889-96. 2002-05-15 [PMID: 11994438] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P01584()