IL-1 beta/IL-1F2 Antibody (43N3D8)

Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.

Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).

Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-P
• Clone: 43N3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

IL-1 beta/IL-1F2 Antibody (43N3D8) Summary

• Immunogen: Full-length recombinant human Interleukin-1 beta protein was used as immunogen for this antibody.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-5ug/ml
  • Flow Cytometry 0.5ug/10^6cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IL-1 beta/IL-1F2 Antibody (43N3D8)

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1beta (Interleukin-1 beta, IL-1) is a potent inflammatory cytokine which has been well studied. Produced as a precursor, especially in myeloid cells and monocytes/macrophages, Interleukin-1 beta is processed by cleavage with caspase-1, or ICE, from the 31 kD inactive precursor to the 18kD active mature form secreted from cells as an end-result of the inflammasome complex and activation sequence. In response to various TLR ligands, myeloid cells or macrophages activate NLRP3 inflammasomes and secrete Interleukin-1 beta. The rate of Interleukin-1 beta processing has been correlated to redox state or levels of ROS generated. Interleukin-1 beta is directly involved in a variety of diseases-auto-inflammatory diseases, acute inflammatory attacks such as ischemic injury in myocardial infarcts or in chronic inflammatory processes of atherosclerosis or Type 2 diabetes. The ability to detect and quantitate active Interleukin-1 beta is critical for understanding how to regulate its activity as a therapeutic target.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (NBP2-27345)(1)

Publication using NBP2-27345

• Giusti I, Monache SD, Di Francesco M et al. From glioblastoma to endothelial cells through extracellular vesicles: messages for angiogenesis. Tumour Biol. 2016 Jul 22 [PMID: 27448307] (WB, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P01584()