IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)

Immunocytochemistry/Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL-1 alpha.

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL-1 alpha (NP_000566) produced in Analysis of HEK293T cell (5 ug, right lane).

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-71021] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free (NBP2-45400) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 30.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for IL-1 alpha/IL-1F1 Antibody (OTI2F8) - Azide and BSA Free

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL1A