IL-1 alpha/IL-1F1 Antibody (3405) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 3405
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

IL-1 alpha/IL-1F1 Antibody (3405) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human IL‑1 alpha /IL‑1F1
  Ser113-Ala271
  Accession # P01583
• Specificity: Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-1 alpha , recombinant rat IL-1 alpha , recombinant cotton rat IL-1 alpha , recombinant human (rh) IL-1 beta or rhIL-18 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1A
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Neutralization
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from ascites

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IL-1 alpha/IL-1F1 Antibody (3405) [Alexa Fluor® 532]

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL1A