IL-1 alpha/IL-1F1 Antibody (3405) [Fluorescein] - Membrane Form

Human peripheral blood mononuclear cells (PBMCs) treated with LPS for 24 hours were stained with Mouse Anti-Human IL-1a/IL-1F1 Membrane Form Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB200F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 3405
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

IL-1 alpha/IL-1F1 Antibody (3405) [Fluorescein] - Membrane Form Summary

• Immunogen: E. coli-derived recombinant human IL‑1 alpha /IL‑1F1
  Ser113-Ala271
  Accession # P01583
• Specificity: Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-1 alpha , recombinant rat IL-1 alpha , recombinant cotton rat IL-1 alpha , recombinant human (rh) IL-1 beta or rhIL-18 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1A
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody (3405) [Fluorescein] - Membrane Form

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha . The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 alpha/IL-1F1 Antibody (FAB200F)(3)

Publications using FAB200F

• SM Ong, E Hadadi, TM Dang, WH Yeap, CT Tan, TP Ng, A Larbi, SC Wong The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence Cell Death Dis, 2018-02-15;9(3):266. 2018-02-15 [PMID: 29449647] (Flow Cytometry, Human)
• Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, Curran S, Davalos A, Wilson-Edell K, Liu S, Limbad C, Demaria M, Li P, Hubbard G, Ikeno Y, Javors M, Desprez P, Benz C, Kapahi P, Nelson P, Campisi J MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol, 2015-07-06;17(8):1049-61. 2015-07-06 [PMID: 26147250] (Flow Cytometry, Human)
• Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha. Eur J Immunol, 2015-01-19;45(4):1092-102. 2015-01-19 [PMID: 25545357] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL1A
• Uniprot:
  • P01583()