IKK epsilon/IKBKE Antibody - BSA Free

Western Blot: IKK epsilon/IKBKE Antibody [NBP1-33052] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: IKK epsilon/IKBKE Antibody [NBP1-33052] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IKBKE protein stained by IKBKE antibody [C2C3], C-term diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody [NBP1-33052] - Paraffin-embedded human endometrium. IKBKE antibody [C2C3], C-term diluted at 1:500.

Immunocytochemistry/Immunofluorescence: IKK epsilon/IKBKE Antibody [NBP1-33052] - Analysis of methanol-fixed HeLa, using IKBKE antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody [NBP1-33052] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IKK epsilon/IKBKE Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IKK epsilon/IKBKE Antibody - BSA Free (NBP1-33052) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human IKK epsilon/IKBKE. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IKBKE
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for IKK epsilon/IKBKE Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase epsilon
• IKBKE
• IKK epsilon
• IKK-E
• IKKEMGC125297
• IKK-epsilon
• IKKI
• IKK-I
• IKK-iMGC125295
• Inducible I kappa-B kinase
• inducible IkappaB kinase
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• inhibitor of nuclear factor kappa-B kinase subunit epsilon
• KIAA0151IKK-related kinase epsilon
• MGC125294

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using a subtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wild type IKKi in cells phosphorylates Ser32 and Ser36 of IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: IKBKE
• Entrez:
  • 9641(Human)
• Uniprot:
  • Q14164()