IKBKAP Antibody - BSA Free

Western Blot: IKBKAP Antibody [NBP1-76788] - A-20 cell lysate with IKBKAP antibody at in (A) 0.5, and (B) 1 u/mL.

Immunocytochemistry/Immunofluorescence: IKBKAP Antibody [NBP1-76788] - A-20 cells with IKBKAP antibody at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: IKBKAP Antibody - BSA Free [NBP1-76788] - Immunofluorescence of IKBKAP in A20 cells with IKBKAP antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IKBKAP Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP. Amino Acid Squence: PPKINRRTQWKLSLLD
• Specificity: At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1-20 ug/mL
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 105, 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IKBKAP Peptide (NBP1-76788PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rabbit (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IKBKAP Antibody - BSA Free

• DYS
• elongator complex protein 1
• ELP1DKFZp781H1425
• FD
• FLJ12497
• IKAPdysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy typeIII)
• IkappaB kinase complex-associated protein
• IKI3
• IKK complex-associated protein
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinasecomplex-associated protein
• p150
• TOT1

Background

IKAP was initially identified as a scaffold protein of the IkappaB kinase complex that could bind to IKKalpha, IKKbeta, NF-kappaB, and the NF-kappaB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELP1
• Uniprot:
  • AAC64258(Human)