IKBKAP Antibody - BSA Free

Western Blot: IKBKAP Antibody [NBP1-71918] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-IKAP antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IKAP was also immunoprecipitated by rabbit anti-IKAP antibody NBP1-71919, which recognizes a downstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IKBKAP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IKBKAP Antibody - BSA Free (NBP1-71918) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1150 and 1200 of human Inhibitor of Kappa Light Polypeptide Gene Enhancer in B-cells, Kiinase Complex-Associated Protein using the numbering given in entry NP_003631.2 (GeneID 8518).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IKBKAP Antibody - BSA Free

• DYS
• elongator complex protein 1
• ELP1DKFZp781H1425
• FD
• FLJ12497
• IKAPdysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy typeIII)
• IkappaB kinase complex-associated protein
• IKI3
• IKK complex-associated protein
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinasecomplex-associated protein
• p150
• TOT1

Background

The transcription factor NF-kappa B coordinates the activation of numerous genes in response to pathogens and pro-inflammatory cytokines, and is, therefore, vital in the development of acute and chronic inflammatory diseases. NF-kappa B is activated by cytokine-activated IKB kinases (IKKs); IKK-alpha and IKK-beta isozymes are found in large complexes. Theses large, interleukin-1-inducible IKK complexes have been found to contain a new protein, termed IKK-complex-associated protein (IKAP), which can bind NIK and IKKs and assemble them into an active kinase complex. IKAP is a scaffold protein and a regulator for 3 different kinases involved in pro-inflammatory cytokine signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELP1
• Uniprot:
  • NP_003631.2(Human)