IkB-alpha [p Ser32, p Ser36] Antibody - BSA Free

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of extracts from Hela cells untreated or treated with TNF using NB100-81987

Immunohistochemistry-Paraffin: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81987(left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/Immunofluorescence: IkB-alpha [p Ser32, p Ser36] Antibody [NB100-81987] - Staining of methanol-fixed Hela cells using NB100-81987

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IkB-alpha [p Ser32, p Ser36] Antibody - BSA Free Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IkB-alpha. around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K).
• Modification: p Ser32, p Ser36
• Specificity: Detects endogenous levels of IkB-alpha only when phosphorylated at serine 32/36.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFKBIA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IkB-alpha [p Ser32, p Ser36] Antibody - BSA Free

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IkB-alpha Antibody (NB100-81987)(2)

Publications using NB100-81987

• Uchida T, Akasaki Y, Sueishi T et al. IkB kinase epsilon contributes to the pathogenesis of osteoarthritis by promoting cartilage degradation Arthritis & rheumatology (Hoboken, N.J.) 2022-12-18 [PMID: 36530063]
• L Song, X Li, Q Sun, Y Zhao Fxyd5 activates the NF?kappaB pathway and is involved in chondrocytes inflammation and extracellular matrix degradation Molecular Medicine Reports, 2022-02-22;25(4):. 2022-02-22 [PMID: 35191523]

Bioinformatics

• Gene Symbol: NFKBIA
• Entrez:
  • 4792(Human)
  • 18035(Mouse)
  • 25493(Rat)
• Uniprot:
  • P25963()