IkB-alpha [p Ser36] Antibody (8G1X10)

Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human colon using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human spleen using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 8G1X10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IkB-alpha [p Ser36] Antibody (8G1X10) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphorylated peptide around S36 of human IkB-alpha (P25963). LDSMK
• Modification: p Ser36
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFKBIA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for IkB-alpha [p Ser36] Antibody (8G1X10)

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIA