IkB-alpha Antibody (116) - Azide and BSA Free

Western Blot: IkB-alpha Antibody (116) [NBP2-90043] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:38 kDa

Immunoprecipitation: IkB-alpha Antibody (116) [NBP2-90043] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg A431 Whole Cell LysateLane D:0.5 mg Hela Whole Cell LysateLane E:0.5 mg K562 Whole Cell Lysate2 uL anti-NFKBIA rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NFKBIA rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 116
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IkB-alpha Antibody (116) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human IkB-alpha (Phe2-Leu317; Accession#: NP_065390.1)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFKBIA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for IkB-alpha Antibody (116) - Azide and BSA Free

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIA