Ikaros/IKZF1 Antibody (4E9) - BSA Free

Flow Cytometry: Mouse Monoclonal Ikaros/IKZF1 Antibody (4E9) [NBP3-09111] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Ikaros (4E9) purified antibody (concentration in sample 8 ug/ml, GAM APC).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IP
• Clone: 4E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ikaros/IKZF1 Antibody (4E9) - BSA Free Summary

• Immunogen: Recombinant human Ikaros/IKZF1 (C-terminal part)
• Specificity: The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IKZF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1-4 ug/ml
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ikaros/IKZF1 Antibody (4E9) - BSA Free

• DNA-binding protein Ikaros
• hIk-1
• IK1
• IK1LyF-1
• Ikaros (zinc finger protein)
• IKAROS family zinc finger 1 (Ikaros)
• Ikaros family zinc finger protein 1
• Ikaros
• IKAROSLymphoid transcription factor LyF-1
• IKZF1
• LYF1
• LyF-1
• LYF1PRO0758
• PRO0758
• zinc finger protein, subfamily 1A, 1 (Ikaros)
• ZNFN1A1
• ZNFN1A1CLL-associated antigen KW-6

Background

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKZF1