Immunohistochemistry-Paraffin: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - IgA2 stained human tonsil FFPE, 20X magnification. Antigen retrieval was performed with citrate pH 6 buffer. Primary antibody at 1:8000. ...read more
Immunohistochemistry: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - Immunohistochemistry of Human Tonsil Tissue using NBP2-62025.
ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - ELISA of human immunoglobulins shows NBP2-62025 only reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with ...read more
ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - A titer ELISA using NBP2-62025. The plate was coated with different amounts of human IgA2. A serial dilution of NBP2-62025 was used as the primary antibody. An ...read more
ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - Sandwich ELISA using NBP2-62025 as the capture antibody, and biotinylated anti-human light chains (kappa+lambda) antibody (NBP2-77396) as the detection ...read more
Antigen retrieval was done with citrate pH6 for 15 mins at 100C and allowed to cool 30 minutes at room temperature. Primary was diluted 1:8000 and incubated at room temperature for 30 mins in Dako antibody diluent (S3022). Secondary ImmPRESS goat anti-rabbit HRP polymer was incubate 30 minutes at room temperature and signal was developed with SIgnalStain DAB chromagen.
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