Rabbit IgG Isotype Control

Images

 
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were ...read more
Flow Cytometry: Rabbit IgG Isotype Control [Azide Free] [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow, ICC/IF, IHC, In vitro
Clonality
Polyclonal
Host
Rabbit
Concentration
0.5 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price
Available Formulations
Catalog# & Formulation Size Price

Rabbit IgG Isotype Control Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ul/1 million cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Application Notes
In vitro assay usage was reported in scientific literature (PMID: 25467444). ICC/IF usage was reported in scientific literature (PMID: 22047818). Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24893 in the following applications:

Publications
Read Publications using
NBP2-24893 in the following applications:

Alternate Names for Rabbit IgG Isotype Control

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for IgG Isotype Control (NBP2-24893)(7)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 4 applications: Flow-CS, ICC/IF, IHC, in vitro.


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Flow-CS
(1)
ICC/IF
(1)
IHC
(1)
in vitro
(1)
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Rat
(1)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-24893 Applications Species
Freund A, Zhong Fl, Venteicher As et al. Proteostatic Control of Telomerase Function through TRiC-Mediated Folding of TCAB1 Cell et al. 2014 Nov 20 [PMID:25467444] (in vitro) in vitro
Shimazu T, Villena J, Tohno M et al. Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infect Immun. 2012 Jan [PMID:22083706] (Flow-CS) Flow-CS
Chiba Eriko, Villena Julio, Hosoya Shoichi et al. A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle. Res Vet Sci. 2012 Oct [PMID:22047818] (ICC/IF) ICC/IF
Hosoya Shoichi, Villena Julio, Shimazu Tomoyuki et al. Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells. Vet Res. 2011 [PMID:22046952] (IHC) IHC
Liebelt B, Papapetrou P, Ali A et al. Exercise preconditioning reduces neuronal apoptosis in stroke by up-regulating heat shock protein-70 (heat shock protein-72) and extracellular-signal-regulated-kinase 1/2. Neuroscience. 2010 Apr 14 [PMID:20083167] (Rat) Rat
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID:19922426]
Patole PS, Pawar RD, Lech M et al. Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice. Nephrol Dial Transplant. 2006 Nov [PMID:16954173]

Reviews for IgG Isotype Control (NBP2-24893) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using NBP2-24893:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation IgG NBP2-24893
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reviewed by:
Anonymous
IP Human 08/26/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedHEK293 cells treated with etoposide (5h)
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

Secondary Descriptionanti rabbit HRP-conjugated antibody
Secondary Manufacturer Cat#(Life Technologies # 65-6120)
Secondary Concentration1:20,000

Details

Detection NotesSuperSignal West Pico Chemiluminescent Substrate (Thermo), 2 min exposure using X-ray film
Immunoprecipitation DetailsProtein G PLUS-Agarose (Santa Cruz), 20ul, 4h at 4 degree, washed thrice with PBS containing 0.1% Triton X-100
Elution DescriptionEluted by boiling in 1X SDS dye for 10 minutes

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG Isotype Control (NBP2-24893) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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