IGFBP-3 Antibody

Western Blot: IGFBP-3 Antibody [NBP1-00203] - (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IGFBP-3 Antibody Summary

• Description: Novus Biologicals Goat IGFBP-3 Antibody (NBP1-00203) is a polyclonal antibody validated for use in WB and ELISA. Anti-IGFBP-3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RYKVDYESQSTDTQN corresponding to internal region according to NP_000589.2.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGFBP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.02 - 0.06 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in human breast cancer lysates (calculated MW of 31.7 kDa band according to NP_001013416.1 and 32.2 kDa band according to NP_000589.2). An additional band of 50 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IGFBP-3 Antibody

• acid stable subunit of the 140 K IGF complex
• binding protein 29
• binding protein 53
• growth hormone-dependent binding protein
• IBP-3
• IBP3BP-53
• IGF-binding protein 3
• IGFBP3
• IGFBP-3
• insulin-like growth factor binding protein 3
• insulin-like growth factor-binding protein 3

Background

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-3 Antibody (NBP1-00203)(1)

Publication using NBP1-00203

• Lin J, Manson JE, Lee IM et al. Intakes of calcium and vitamin D and breast cancer risk in women. Arch Intern Med 2007-05-28 [PMID: 17533208]

Bioinformatics

• Gene Symbol: IGFBP3
• Entrez:
  • 3486(Human)
  • 16009(Mouse)
  • 24484(Rat)
• Uniprot:
  • NP_001013416.1(Human)