IGFBP-3 Antibody (84728) [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, IHC, ELISA, ELISA(Cap), ELISA(Det)
• Clone: 84728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 669

IGFBP-3 Antibody (84728) [Janelia Fluor® 669] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IGFBP‑3
  Gly28-Lys291
  Accession # CAA46087
• Specificity: Detects human IGFBP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) IGF‑I R, rhIGFBP-1, rhIGFBP-2, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IGFBP-3 Antibody (84728) [Janelia Fluor® 669]

• acid stable subunit of the 140 K IGF complex
• binding protein 29
• binding protein 53
• growth hormone-dependent binding protein
• IBP-3
• IBP3BP-53
• IGF-binding protein 3
• IGFBP3
• IGFBP-3
• insulin-like growth factor binding protein 3
• insulin-like growth factor-binding protein 3

Background

IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP3