IGFBP-3 Antibody (84728) [Unconjugated]

IGFBP-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human IGFBP-3 Monoclonal Antibody (Catalog # MAB305) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

The effect of tumour carriage on skeletal muscle Skeletal-muscle samples were obtained from normal athymic mice (group N, n = 14) and from those that carried MiaPaCa2 (group M, n = 10) or AsPC1 cells (group A, n = 13). Western blots were performed to determine atrogin-1 (a), IGFBP-3 (a), myosin (b), and MURF1 (b), using beta -tubulin as a loading control. The blots are representative data. The histograms show the results of all mice (See n in the parentheses). *P < 0.05, NS: not significant Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4271-3), licensed under a CC-BY license. Not internally tested by R&D Systems.

The effect of tumour carriage on skeletal muscle Skeletal-muscle samples were obtained from normal athymic mice (group N, n = 14) and from those that carried MiaPaCa2 (group M, n = 10) or AsPC1 cells (group A, n = 13). Western blots were performed to determine atrogin-1 (a), IGFBP-3 (a), myosin (b), and MURF1 (b), using beta -tubulin as a loading control. The blots are representative data. The histograms show the results of all mice (See n in the parentheses). *P < 0.05, NS: not significant Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29609556), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Cap)
• Clone: 84728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IGFBP-3 Antibody (84728) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IGFBP‑3
  Gly28-Lys291
  Accession # CAA46087
• Specificity: Detects human IGFBP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) IGF‑I R, rhIGFBP-1, rhIGFBP-2, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human IGFBP-3 Biotinylated Antibody (Catalog number BAF675)
  Standard: Recombinant Human IGFBP-3 (Catalog number 675-B3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-3 Antibody (84728) [Unconjugated]

• acid stable subunit of the 140 K IGF complex
• binding protein 29
• binding protein 53
• growth hormone-dependent binding protein
• IBP-3
• IBP3BP-53
• IGF-binding protein 3
• IGFBP3
• IGFBP-3
• insulin-like growth factor binding protein 3
• insulin-like growth factor-binding protein 3

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked and two potential O-linked glycosylation sites. Human IGFBP-3 is expressed in multiple tissues. The highest expression level is found in the non-paranchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty. Human IGFBP-3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).

1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
3. Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-3 Antibody (MAB305)(16)

Publications using MAB305

• Chen, YI;Tien, SC;Ko, YL;Chang, CC;Hsu, MF;Chien, HJ;Peng, HY;Jeng, YM;Tien, YW;Chang, YT;Chang, MC;Hu, CM; SEMA7A-mediated juxtacrine stimulation of IGFBP-3 upregulates IL-17RB at pancreatic cancer invasive front Cancer gene therapy 2024-10-24 [PMID: 39448803] (Immunocytochemistry, Human)
• Hasuike Y, Mochizuki H, Nakamori M. Expanded CUG Repeat RNA Induces Premature Senescence in Myotonic Dystrophy Model Cells Frontiers in Genetics 2022-03-25 [PMID: 35401669]
• Hasuike Y, Mochizuki H, Nakamori M. Expanded CUG Repeat RNA Induces Premature Senescence in Myotonic Dystrophy Model Cells Frontiers in Genetics 3/25/2022 [PMID: 35401669]
• HJ Shih, HF Chang, CL Chen, PL Torng Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer Scientific Reports, 2021-11-25;11(1):22925. 2021-11-25 [PMID: 34824343] (ICC, IHC, Neutralization, Human, Xenograft)
• J Sakata, A Hirosue, R Yoshida, Y Matsuoka, K Kawahara, H Arita, H Nakashima, T Yamamoto, M Nagata, S Kawaguchi, S Gohara, Y Nagao, K Yamana, R Toya, R Murakami, Y Kuwahara, M Fukumoto, H Nakayama Enhanced Expression of IGFBP-3 Reduces Radiosensitivity and Is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma Cancers (Basel), 2020-02-20;12(2):. 2020-02-20 [PMID: 32093285] (IHC, Human)
• Rossella Titone, Meifang Zhu, Danielle M. Robertson Mutual regulation between IGF‐1R and IGFBP‐3 in human corneal epithelial cells Journal of Cellular Physiology 2/1/2019 [PMID: 30078228]
• Rossella Titone, Meifang Zhu, Danielle M. Robertson Mutual regulation between IGF‐1R and IGFBP‐3 in human corneal epithelial cells Journal of Cellular Physiology 2019-02-01 [PMID: 30078228] (Western Blot, Human)
• Watanabe K, Uemura K, Asada M, Maesako M, Akiyama H, Shimohama S, Takahashi R, Kinoshita A The participation of insulin-like growth factor-binding protein 3 released by astrocytes in the pathology of Alzheimer&#039;s disease. Mol Brain, 2015-12-04;8(1):82. 2015-12-04 [PMID: 26637371] (Western Blot, Human)
• Yi-Chen Yen, Jenn-Ren Hsiao, Shih Sheng Jiang, Jeffrey S. Chang, Ssu-Han Wang, Ying-Ying Shen et al. Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin beta 1 Oncotarget 12/8/2015 [PMID: 26540630]
• Natsuizaka M, Kinugasa H, Kagawa S, Whelan K, Naganuma S, Subramanian H, Chang S, Nakagawa K, Rustgi N, Kita Y, Natsugoe S, Basu D, Gimotty P, Klein-Szanto A, Diehl J, Nakagawa H IGFBP3 promotes esophageal cancer growth by suppressing oxidative stress in hypoxic tumor microenvironment. Am J Cancer Res, 2014-01-15;4(1):29-41. 2014-01-15 [PMID: 24482736] (IHC, Human)
• David B. Seligson, Hong Yu, Sheila Tze, Jonathan Said, Allan J. Pantuck, Pinchas Cohen et al. IGFBP-3 Nuclear Localization Predicts Human Prostate Cancer Recurrence Hormones and Cancer 2/1/2013 [PMID: 23011725]
• David B. Seligson, Hong Yu, Sheila Tze, Jonathan Said, Allan J. Pantuck, Pinchas Cohen et al. IGFBP-3 Nuclear Localization Predicts Human Prostate Cancer Recurrence Hormones and Cancer 2013-02-01 [PMID: 23011725] (Immunohistochemistry, Human)
• Elzi , David J, Lai , Yanlai, Song , Meihua, Hakala , Kevin, Weintraub , Susan T, Shiio , Yuzuru Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence. Proc Natl Acad Sci U S A, 2012-07-09;109(30):12052-7. 2012-07-09 [PMID: 22778398] (IHC-P, Human)
• TSUNG-FAN TUAN, CHENG-TA CHUNG, HSIAO-HUI TSOU, FONG-WEN CHEN, HENG-LIANG LIN, YIU-KAY LAI et al. Putative tumor metastasis-associated genes in human gastric cancer International Journal of Oncology 9/1/2012 [PMID: 22664961]
• Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion. Endocrinology, 2011-03-29;152(6):2184-96. 2011-03-29 [PMID: 21447640] (IHC-P, Western Blot, Mouse)
• Takaoka M, Harada H, Andl CD, Oyama K, Naomoto Y, Dempsey KL, Klein-Szanto AJ, El-Deiry WS, Grimberg A, Nakagawa H Epidermal growth factor receptor regulates aberrant expression of insulin-like growth factor-binding protein 3. Cancer Res., 2004-11-01;64(21):7711-23. 2004-11-01 [PMID: 15520175] (ICC, IHC-P, Human)

Bioinformatics

• Gene Symbol: IGFBP3
• Uniprot:
  • CAA46087()