IGFBP-2 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IGFBP-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IGFBP‑2
  Glu35-Gln305
  Accession # CAA57270
• Specificity: Detects mouse IGFBP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) IGFBP-2 is observed and less than 1% cross-reactivity with rhIGFBP-1, rhIGFBP-3, rhIGFBP-4, rhIGFBP-5, recombinant mouse (rm) IGFBP-3, rmIGFBP-4, rmIGFBP-5, and rmIGFBP-6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGFBP2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 30 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-2 Antibody [Unconjugated]

• BP2
• IBP2
• IBP-2
• IGF-binding protein 2
• IGFBP2
• IGFBP-2
• IGF-BP53
• insulin-like growth factor binding protein 2 (36kD)
• insulin-like growth factor binding protein 2, 36kDa
• insulin-like growth factor-binding protein 2

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) residue precursor protein with a putative 34 aa residue signal peptide that is processed to generate the 271 aa residue mature protein. Mouse and human IGFBP-2 share approximately 82% homology. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF-II, exhibiting a 2-10 fold higher affinity for IGF-II than for IGF-I.

1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
3. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
4. Landwehr, J. et al. (1993) Gene 124:281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-2 Antibody (AF797)(5)

Publications using AF797

• Alicea, GM;Portuallo, ME;Patel, P;Fane, ME;Carey, AE;Speicher, D;Tang, HY;Kossenkov, AV;Rebecca, VW;Wirtz, DG;Weeraratna, AT; Age-related increases in IGFBP2 increase melanoma cell invasion and lipid synthesis bioRxiv : the preprint server for biology 2023-05-02 [PMID: 37205503] (In Vivo, Mouse)
• ALM Caldwell, L Sancho, J Deng, A Bosworth, A Miglietta, JK Diedrich, MN Shokhirev, NJ Allen Aberrant astrocyte protein secretion contributes to altered neuronal development in multiple models of neurodevelopmental disorders Nature Neuroscience, 2022-08-30;25(9):1163-1178. 2022-08-30 [PMID: 36042312] (IHC, Mouse)
• Fletcher L, Isgor E, Sprague S, Williams L, Alajajian B, Jimenez D, Digicaylioglu M Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury. BMC Neurosci, 2013-12-21;14(0):158. 2013-12-21 [PMID: 24359611] (ICC, IHC, Mouse, Rat)
• Decreased expression of extracellular matrix proteins and trophic factors in the amygdala complex of depressed mice after chronic immobilization stress. BMC Neurosci, 2012-06-06;13(0):58. 2012-06-06 [PMID: 22672618] (IHC, Mouse)
• Lee J, Basak JM, Demehri S, Kopan R Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes. Development, 2007-07-04;134(15):2795-806. 2007-07-04 [PMID: 17611229] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: IGFBP2
• Uniprot:
  • CAA57270()