IGFBP-2 Antibody (CL13563) - Azide and BSA Free

Staining of human anaplastic oligodendroglioma shows strong membranous positivity in tumor cells.

Analysis in human pancreas and skin tissues using NBP3-43841 antibody. Corresponding IGFBP-2 RNA-seq data are presented for the same tissues.

Staining of human endometrial cancer shows strong membranous positivity in tumor cells.

Staining of human skin shows negative to weak positivity in squamous epithelial cells.

Staining of human ovarian cancer (low grade serous ovarian carcinoma) shows strong membranous positivity in tumor cells.

Staining of human ovarian cancer (papillary carcinoma) shows strong membranous positivity in tumor cells.

Staining of human pancreas shows strong membranous positivity in exocrine glandular cells.

Staining of human stomach shows strong membranous positivity in glandular cells.

Analysis in human cell line NB4.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13563
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IGFBP-2 Antibody (CL13563) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IGFBP-2 Antibody (CL13563) - Azide and BSA Free (NBP3-24568) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P18065, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for IGFBP-2 Antibody (CL13563) - Azide and BSA Free

• BP2
• IBP2
• IBP-2
• IGF-binding protein 2
• IGFBP2
• IGFBP-2
• IGF-BP53
• insulin-like growth factor binding protein 2 (36kD)
• insulin-like growth factor binding protein 2, 36kDa
• insulin-like growth factor-binding protein 2

Background

IGFBP2 is a member of the ISGBP family which bind various IGFs. IGFBP2 is over expressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The over expression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP2