IGF-II R/IGF2R Antibody (SR45-09)

Western Blot: IGF-II R/IGF2R Antibody (SR45-09) [NBP2-67347] - Western blot analysis of IGF-II R/IGF2R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (SR45-09) [NBP2-67347] - Staining M6PR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IGF-II R/IGF2R Antibody (SR45-09) [NBP2-67347] - Analysis of paraffin-embedded human tonsil tissue using anti-M6PR antibody. Counter stained with hematoxylin.

Flow Cytometry: IGF-II R/IGF2R Antibody (SR45-09) [NBP2-67347] - Analysis of Hela cells with M6PR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (SR45-09) [NBP2-67347] - Staining M6PR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (SR45-09) [NBP2-67347] - Staining M6PR in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

IGF-II R/IGF2R Antibody (SR45-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human IGF-II R/IGF2R aa 2,442-2,491 / 2,491. (SwissProt: P11717 Human; SwissProt: Q07113 Mouse; Entrez Gene: 25151 Rat)
• Localization: Golgi apparatus membrane, Endosome membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGF2R
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IGF-II R/IGF2R Antibody (SR45-09)

• 300 kDa mannose 6-phosphate receptor
• cation-independent mannose-6 phosphate receptor
• cation-independent mannose-6-phosphate receptor
• CD222 antigen
• CD222
• CI Man-6-P receptor
• CIMPR
• CI-MPR
• IGF2R
• IGF-II R
• IGF-II receptor
• IGFIIR
• IGF-IIR
• insulin-like growth factor 2 receptorM6P/IGF2R
• Insulin-like growth factor II receptor
• M6P/IGF2 receptor
• M6PR
• M6P-R
• MPR 300
• MPR1
• MPRI
• MPRIM6PR

Background

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGF2R