IGF-I R/IGF1R Antibody (S07-7E1)

Western Blot: IGF-I R/IGF1R Antibody (S07-7E1) [NBP3-14897] - Western blot detection of IGF-I R/IGF1R in Hela, A549, HL-60, U2OS cell lysates using IGF-I R/IGF1R Rabbit mAb (1:1000 diluted). Predicted band size: 155kDa. Observed band size: 95kDa.

Immunohistochemistry: IGF-I R/IGF1R Antibody (S07-7E1) [NBP3-14897] - Immunohistochemical of IGF-I R/IGF1R in Human lung cancer tissue using IGF-I R/IGF1R antibody at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S07-7E1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IGF-I R/IGF1R Antibody (S07-7E1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human IGF-I R/IGF1R (Uniprot#: P08069)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGF1R
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for IGF-I R/IGF1R Antibody (S07-7E1)

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGF1R