IFN-gamma R2 Antibody [Unconjugated]

Blood-derived granulocytes were stained with Goat Anti-Human IFN‑ gamma R2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF773, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

B3GNT2 disrupts ligand–receptor interactions between tumor and T cells.a Cell survival against T cell cytotoxicity (top) and T cell IFN gamma secretion (bottom) in A375 cells overexpressing B3GNT2 or GFP that have been treated with different concentrations of kifunensine. Kifunensine was used to pretreat A375 cells and was present during co-culture with T cells at E:T ratio of 3. Kifunensine-treated cells that were co-cultured with ESO T cells were compared to kifunensine-treated cells cultured in parallel without T cells to determine percent survival. N = 6. Two-tailed t tests were performed. b Tomato lectin IP of A375 cells overexpressing GFP or B3GNT2 followed by western blot for different B3GNT2 target proteins. 2% of the input and no lectin IP controls are shown. Data are representative of two independent experiments. c Western blots of A375 cells overexpressing B3GNT2 or GFP that were treated with kifunensine (KIF) or benzyl-2-acetamido-2-deoxy-alpha -D-galactopyranoside (BAG) to remove N- or O-glycosylation respectively. Data are representative of two independent experiments. d Histograms (top) and corresponding median fluorescence intensity (MFI; bottom) showing binding of recombinant T cell proteins to A375 cells measured by flow cytometry. A375 cells were overexpressing GFP or B3GNT2 and treated with KIF or BAG. N = 3. Two-tailed t tests were performed. e Schematic showing the tumor cell surface ligands and receptors modified by B3GNT2 to disrupt interactions with T cells that mediate cytotoxicity. All values are mean ± s.e.m. ns not significant. Source data are provided in Source Data 4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35338135), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human IFN‑ gamma R2 Antibody (Catalog # AF773) neutralizes IFN‑ gamma R2-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND50) is typically <25.0 µg/mL in the presence of Recombinant Human IFN‑ gamma .

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFN-gamma R2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
  Ala22-Gln247
  Accession # P38484
• Specificity: Detects human IFN-gamma R2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNGR2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R2 Antibody [Unconjugated]

• AF-1
• AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
• IFGR2
• IFN-gamma R2
• IFN-gamma receptor 2
• IFNgammaR2
• IFN-gamma-R2
• IFNGR2
• IFN-gR2
• IFNGT1
• interferon gamma receptor 2 (interferon gamma transducer 1)
• interferon gamma receptor 2
• interferon gamma receptor accessory factor-1
• interferon gamma receptor beta chain
• Interferon gamma transducer 1

Background

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma . IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma R2 Antibody (AF773)(5)

Publications using AF773

• Khanolkar, A;Liu, G;Simpson Schneider, BM; Defining the Basal and Immunomodulatory Mediator-Induced Phosphoprotein Signature in Pediatric B Cell Acute Lymphoblastic Leukemia (B-ALL) Diagnostic Samples International journal of molecular sciences 2023-09-11 [PMID: 37762241] (Flow Cytometry, Human)
• J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-03-25;13(1):1606. 2022-03-25 [PMID: 35338135] (Western Blot, Human)
• CM Blouin, Y Hamon, P Gonnord, C Boularan, J Kagan, C Viaris de, R Ruez, S Mailfert, N Bertaux, D Loew, C Wunder, L Johannes, G Vogt, FX Contreras, D Marguet, JL Casanova, C Galès, HT He, C Lamaze Glycosylation-Dependent IFN-?R Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation Cell, 2016-08-04;166(4):920-34. 2016-08-04 [PMID: 27499022] (Western Blot, Human)
• Trisomy 21 consistently activates the interferon response Elife, 2016-07-29;5(0):. 2016-07-29 [PMID: 27472900] (Western Blot, Human)
• Lee WI, Liang FC, Huang JL, Jaing TH, Wang CH, Lin TY, Huang YC, Huang WL, Jou R, Hsieh MY, Chia JH, Wu TL Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease. J. Clin. Immunol., 2008-12-13;29(3):319-29. 2008-12-13 [PMID: 19089604] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IFNGR2
• Uniprot:
  • P38484()