IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]

Images

 
Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
92101
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

IFN-gamma R1/CD119 Antibody (92101) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1
Glu18-Gly245
Accession # P15260.1
Specificity
Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma  R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFNGR1
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 0.01-0.03 ug/mL
Control
IFN-gamma R1/CD119 Knockout HeLa Cell Lysate
Publications
Read Publications using
MAB6731 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R1/CD119 Antibody (92101) [Unconjugated]

  • AVP, type 2
  • CD119 antigen
  • CD119
  • FLJ45734
  • IFN-gamma R1
  • IFN-gamma receptor 1
  • IFNgammaR1
  • IFN-gamma-R1
  • IFNGR
  • IFNGR1
  • IFN-gR1
  • immune interferon receptor 1
  • interferon gamma receptor 1
  • interferon-gamma receptor alpha chain
  • type 2

Background

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFN-gamma R1/CD119 Antibody (MAB6731)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, Neutralization, Neutralizing.


Filter By Application
Flow Cytometry
(2)
Neutralization
(5)
Neutralizing
(1)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB6731 Applications Species
PA Szabo, HM Levitin, M Miron, ME Snyder, T Senda, J Yuan, YL Cheng, EC Bush, P Dogra, P Thapa, DL Farber, PA Sims Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease Nat Commun, 2019;10(1):4706. 2019 [PMID: 31624246] (Neutralization, Human) Neutralization Human
L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum J. Allergy Clin. Immunol., 2018;0(0):. 2018 [PMID: 29371118] (Neutralizing, Human) Neutralizing Human
C Xia, JJ Wolf, M Vijayan, CJ Studstill, W Ma, B Hahm Casein kinase 1? mediates degradation of receptors for type I and type II interferons caused by hemagglutinin of influenza A virus J. Virol., 2018;0(0):. 2018 [PMID: 29343571] (Flow Cytometry, Human) Flow Cytometry Human
SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing PLoS ONE, 2017;12(8):e0183390. 2017 [PMID: 28837681] (Neutralization, Human) Neutralization Human
LM Feldhoff, CM Rueda, ME Moreno-Fer, J Sauer, CM Jackson, CA Chougnet, J Rupp IL-1? induced HIF-1? inhibits the differentiation of human FOXP3(+) T cells Sci Rep, 2017;7(1):465. 2017 [PMID: 28352109] (Neutralization, Human) Neutralization Human
Love A, Schwartz I, Petzke M Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential. J Leukoc Biol, 2015;97(2):379-90. 2015 [PMID: 25420916] (Neutralization, Human) Neutralization Human
Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway. J. Cell. Physiol., 2007;210(1):192-200. 2007 [PMID: 17013806] (Neutralization, Human) Neutralization Human
Ebert EC, Mehta V Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006;241(1):7-13. 2006 [PMID: 16914126] (Flow Cytometry, Human) Flow Cytometry Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6731U
Alexa Fluor 405 FAB6731V
Alexa Fluor 488 FAB6731G
Alexa Fluor 594 FAB6731T
Alexa Fluor 647 FAB6731R
Alexa Fluor 700 FAB6731N
Alexa Fluor 750 FAB6731S
Fluorescein FAB673F
Phycoerythrin FAB673P

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Bioinformatics

Gene Symbol IFNGR1
Uniprot