IFN-gamma R2 Antibody [Allophycocyanin]

Human peripheral blood granulocytes were stained with Goat Anti-Human IFN-gamma R2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB773A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

IFN-gamma R2 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
  Ala22-Gln247
  Accession # P38484
• Specificity: Detects human IFN-gamma R2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human IFN‑ gamma R1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNGR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R2 Antibody [Allophycocyanin]

• AF-1
• AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
• IFGR2
• IFN-gamma R2
• IFN-gamma receptor 2
• IFNgammaR2
• IFN-gamma-R2
• IFNGR2
• IFN-gR2
• IFNGT1
• interferon gamma receptor 2 (interferon gamma transducer 1)
• interferon gamma receptor 2
• interferon gamma receptor accessory factor-1
• interferon gamma receptor beta chain
• Interferon gamma transducer 1

Background

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma . IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma R2 Antibody (FAB773A)(8)

Publications using FAB773A

• Aiko Hirayama, Kentaro Tanaka, Hirono Tsutsumi, Takayuki Nakanishi, Sho Yamashita, Shun Mizusaki et al. Regulation of PD-L1 expression in non–small cell lung cancer by interleukin-1 beta Frontiers in Immunology 6/27/2023 [PMID: 37441079]
• Aiko Hirayama, Kentaro Tanaka, Hirono Tsutsumi, Takayuki Nakanishi, Sho Yamashita, Shun Mizusaki et al. Regulation of PD-L1 expression in non–small cell lung cancer by interleukin-1 beta Frontiers in Immunology 2023-06-27 [PMID: 37441079] (Flow Cytometry, Human)
• C Lundtoft, P Pucholt, J Imgenberg-, J Carlsson-A, ML Eloranta, AC Syvänen, G Nordmark, JK Sandling, I Kockum, T Olsson, L Rönnblom, N Hagberg Function of multiple sclerosis-protective HLA class I alleles revealed by genome-wide protein-quantitative trait loci mapping of interferon signalling PLoS Genet, 2020-10-26;16(10):e1009199. 2020-10-26 [PMID: 33104735] (Flow Cytometry, Human)
• Xiao-Fei Kong, Lisa Worley, Darawan Rinchai, Vincent Bondet, Puthen Veettil Jithesh, Marie Goulet et al. Three Copies of Four Interferon Receptor Genes Underlie a Mild Type I Interferonopathy in Down Syndrome Journal of Clinical Immunology 8/1/2020 [PMID: 32572726]
• Sara Abouelasrar Salama, Mieke Gouwy, Alexandra De Zutter, Noëmie Pörtner, Lotte Vanbrabant, Nele Berghmans et al. Induction of Chemokines by Hepatitis C Virus Proteins: Synergy of the Core Protein with Interleukin-1 beta and Interferon-gamma in Liver Bystander Cells Journal of Interferon & Cytokine Research 4/1/2020 [PMID: 32031878]
• Li J, Chen J, Zeng T, He F, Chen S, Ma S, Bi J, Zhu X, Guan X CD133+ liver cancer stem cells resist interferon-gamma-induced autophagy. BMC Cancer, 2016-01-13;16(1):15. 2016-01-13 [PMID: 26758620] (Flow Cytometry, Human)
• Marcela Moncada-Vélez, Rubén Martinez-Barricarte, Dusan Bogunovic, Xiao-Fei Kong, Lizbeth Blancas-Galicia, Cengiz Tirpan et al. Partial IFN-gamma R2 deficiency is due to protein misfolding and can be rescued by inhibitors of glycosylation Blood 10/3/2013 [PMID: 23963039]
• de Paus RA, Kilic SS, Van Dissel JT, van de Vosse E Effect of amino acid substitutions in the human IFN-gammaR2 on IFN-gamma responsiveness. Genes Immun., 2011-01-20;12(2):136-44. 2011-01-20 [PMID: 21248774] (Flow Cytometry, Human)

Review for IFN-gamma R2 Antibody (FAB773A) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 12/11/2016

Summary

• Application: Flow Cytometry
• Sample Tested: Blood mononuclear cells (PBMCs)
• Species: Human
• Lot: AAZY0313041

Comments

• Comments: Staining is acceptable but have to be used at very high concentrations.

Bioinformatics

• Gene Symbol: IFNGR2
• Uniprot:
  • P38484()