IFN-gamma Antibody

Western Blot: IFN-gamma Antibody [NBP1-77880] - Shows detection of recombinant chicken IFN gamma at 16.7 kDa, raised in yeast. Primary antibody was diluted to 1ug /mL. 3% BSA from BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody was used at 1:20,000.

Product Discontinued

IFN-gamma Antibody Summary

• Description: This product was protein A purified from monospecific antiserum by chromatography
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This protein A purified IFN-gamma Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 145 amino acids of mature chicken IFN-gamma. (Uniprot: D3GGX1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFNG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Western Blot 1:500-1:2000
• Application Notes: This protein A purified This product has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.7 kDa in size corresponding to chicken IFN gamma by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with IFN-gamma from chicken based on 100% homology; ring-necked pheasant, turkey, quail, and junglefowl based on 91-97% homology with the immunizing sequence. Cross-reactivity with IFN-gamma from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Notes

This product was protein A purified from monospecific antiserum by chromatography

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for IFN-gamma Antibody

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IFN-gamma Antibody (NBP1-77880). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • D3GGX1(Human)
  • NC_000012.11(Human)