IFN-gamma Antibody (K3.53) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with either (A) Mouse Anti-Human IFN- gamma Monoclonal Antibody (Catalog # MAB2852) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).View our protocol for Staining Intracellular Molecules.

Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB2852). The ND50 is typically ≤2 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap), Flow
• Clone: K3.53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-gamma Antibody (K3.53) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IFN-gamma
• Specificity: Detects human IFN-gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse IFN-gamma , recombinant rat IFN-gamma , recombinant porcine IFN-gamma , recombinant feline IFN-gamma , or recombinant cotton rat IFN-gamma is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization ≤2 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human IFN-gamma Biotinylated Antibody (Catalog number BAF285) Standard: Recombinant Human IFN-gamma (Catalog number 285-IF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody (K3.53) [Unconjugated]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN- gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up-regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (MAB2852)(20)

Publications using MAB2852

• KJ Roberts, MF Cubitt, TM Carlton, L Rodrigues-, L Maggiore, R Chai, S Clare, K Harcourt, TT MacDonald, KP Ray, A Vossenkämp, MR West, JS Crowe Preclinical development of a bispecific TNFalpha/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease Scientific Reports, 2021-09-30;11(1):19422. 2021-09-30 [PMID: 34593832] (ELISA Capture, Human)
• D Maura, N Elmekki, CA Goddard The ammonia oxidizing bacterium Nitrosomonas eutropha blocks T helper 2 cell polarization via the anti-inflammatory cytokine IL-10 Scientific Reports, 2021-07-08;11(1):14162. 2021-07-08 [PMID: 34238943] (Neutralization, Human)
• Wai Po Chong, Mary J. Mattapallil, Kumarkrishna Raychaudhuri, So Jin Bing, Sihan Wu, Yajie Zhong et al. The Cytokine IL-17A Limits Th17 Pathogenicity via a Negative Feedback Loop Driven by Autocrine Induction of IL-24 Immunity 8/1/2020 [PMID: 32673565]
• A Stumpf, T Brandstett, J Hübner, J Rühe Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum PLoS ONE, 2019-12-02;14(12):e0225525. 2019-12-02 [PMID: 31790441] (ELISA Capture, Human)
• J Hrdý, K Vlasáková, V ?erný, L Súkeníková, O Novotná, P Petrásková, K Boráková, R Lodinová-Ž, L Kolá?ová, L Prokešová Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action Eur. J. Immunol., 2018-10-21;0(0):. 2018-10-21 [PMID: 30306557] (Human)
• J Jhun, SH Lee, SK Lee, HY Kim, ES Jung, DG Kim, J Choi, SH Bae, SK Yoon, BH Chung, CW Yang, ML Cho, JY Choi Serial Monitoring of Immune Markers Being Represented Regulatory T Cell/T Helper 17 Cell Ratio: Indicating Tolerance for Tapering Immunosuppression after Liver Transplantation Front Immunol, 2018-03-01;9(0):352. 2018-03-01 [PMID: 29545795] (ELISA Capture, Human)
• AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways PLoS Pathog., 2017-09-01;13(9):e1006577. 2017-09-01 [PMID: 28863187] (Neutralization, Human)
• E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma PLoS ONE, 2017-04-14;12(4):e0175712. 2017-04-14 [PMID: 28410380] (ELISA Development (Capture), Human)
• Gonzalez-Lombana C, Gimblet C, Bacellar O, Oliveira W, Passos S, Carvalho L, Goldschmidt M, Carvalho E, Scott P IL-17 mediates immunopathology in the absence of IL-10 following Leishmania major infection. PLoS Pathog, 2013-03-21;9(3):e1003243. 2013-03-21 [PMID: 23555256] (Functional Assay, Human)
• Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection. J. Immunol., 2011-06-10;187(2):748-59. 2011-06-10 [PMID: 21666058] (Neutralization, Human)
• Sonemany Salinthone, Vijayshree Yadav, Robynn V. Schillace, Dennis N. Bourdette, Daniel W. Carr Lipoic Acid Attenuates Inflammation via cAMP and Protein Kinase A Signaling PLoS ONE 2010-09-28 [PMID: 20927401] (ELISA Capture, Human)
• Sonemany Salinthone, Vijayshree Yadav, Robynn V. Schillace, Dennis N. Bourdette, Daniel W. Carr Lipoic Acid Attenuates Inflammation via cAMP and Protein Kinase A Signaling PLoS ONE 9/28/2010 [PMID: 20927401]
• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)
• Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-11-01;53(11):2010-2. 2007-11-01 [PMID: 18030696] (ELISA Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human)
• Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (ELISA Development, Human)
• Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Flow Cytometry, Human)
• Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-01-01;284(1):99-106. 2004-01-01 [PMID: 14736420] (Array Development, Human)

FAQs for IFN-gamma Antibody (MAB2852). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG