IFN-gamma Antibody (88928) [Unconjugated]

IFN-gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Mouse Anti-Rat IFN-gamma Monoclonal Antibody (Catalog # MAB5851) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Rt
• Applications: ELISA(Cap), ICC/IF
• Clone: 88928
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-gamma Antibody (88928) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat IFN-gamma
  Gln23-Cys156
  Accession # P01581
• Specificity: Detects rat IFN-gamma in ELISAs. In sandwich ELISAs, observed cross-reactivities were less than 2% with recombinant mouse IFN‑ gamma and less than 0.1% with recombinant human IFN‑ gamma and recombinant porcine IFN‑ gamma .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
• Application Notes: ELISA Detection: Rat IFN-gamma Biotinylated Antibody (Catalog number BAF585)
  Standard: Recombinant Rat IFN-gamma (Catalog number 585-IF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody (88928) [Unconjugated]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37% - 45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma . IFN-gamma dimers bind to IFN‑ gamma  RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Dijkema, R. et al. (1985) EMBO J. 4:761.
4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (MAB5851)(5)

Publications using MAB5851

• T Balzano, P Leone, G Ivaylova, MC Castro, L Reyes, C Ramón, M Malaguarne, M Llansola, V Felipo Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines, 2021-08-12;9(8):. 2021-08-12 [PMID: 34440206] (Western Blot, Rat)
• Junko NOGUCHI, Mitsumi IKEDA, Kazuhiro KIKUCHI, Thanh Quang DANG-NGUYEN, Megumi KASASHIMA, Ken-ichiro TATEMATSU et al. Successful activation of rat T lymphocytes by sperm specific antigens <i>in vitro</i> Journal of Reproduction and Development 12/22/2020 [PMID: 33012735]
• Junko NOGUCHI, Mitsumi IKEDA, Kazuhiro KIKUCHI, Thanh Quang DANG-NGUYEN, Megumi KASASHIMA, Ken-ichiro TATEMATSU et al. Successful activation of rat T lymphocytes by sperm specific antigens <i>in vitro</i> Journal of Reproduction and Development 2020-12-22 [PMID: 33012735] (ELISA Capture, Rat)
• Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells, 2008-01-10;26(4):1047-55. 2008-01-10 [PMID: 18192235] (ELISA Development, Rat)
• Roche M, Diamond M, Finn DP In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. J. Neuroimmunol., 2006-09-29;181(1):57-67. 2006-09-29 [PMID: 17011047] (ELISA Development, Rat)

FAQs for IFN-gamma Antibody (MAB5851). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • P01581()